Detailed information NTG0911172

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U0542_RS14800
• Genome accession: NZ_CP139831
• Coordinates: 2908491..2909609 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain Nord12-3
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2903491..2914609

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0542_RS14770 (U0542_14770)	-	2904320..2905168 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
U0542_RS14775 (U0542_14775)	-	2905421..2905876 (+)	456	WP_000782976.1	bacteriohemerythrin	-
U0542_RS14780 (U0542_14780)	-	2906029..2907096 (+)	1068	WP_000092403.1	hypothetical protein	-
U0542_RS14785 (U0542_14785)	-	2907096..2907419 (+)	324	WP_000442585.1	RnfH family protein	-
U0542_RS14790 (U0542_14790)	-	2907474..2907872 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
U0542_RS14795 (U0542_14795)	fur	2907984..2908421 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
U0542_RS14800 (U0542_14800)	pilU	2908491..2909609 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U0542_RS14805 (U0542_14805)	pilT	2909637..2910674 (-)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
U0542_RS14810 (U0542_14810)	-	2910801..2911493 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=911172 U0542_RS14800 WP_000347036.1 2908491..2909609(-) (pilU) [Acinetobacter baumannii strain Nord12-3]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=911172 U0542_RS14800 WP_000347036.1 2908491..2909609(-) (pilU) [Acinetobacter baumannii strain Nord12-3]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392