Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RA957_RS12710 Genome accession   NZ_CP133077
Coordinates   2515553..2516626 (-) Length   357 a.a.
NCBI ID   WP_011231917.1    Uniprot ID   Q5KX66
Organism   Geobacillus kaustophilus strain T-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2510553..2521626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA957_RS12670 (RA957_12670) - 2511280..2512074 (+) 795 WP_011231909.1 YqhG family protein -
  RA957_RS12675 (RA957_12675) - 2512177..2512356 (-) 180 WP_011231910.1 YqzE family protein -
  RA957_RS12680 (RA957_12680) comGG 2512403..2512792 (-) 390 WP_047758063.1 competence type IV pilus minor pilin ComGG -
  RA957_RS12685 (RA957_12685) comGF 2512867..2513313 (-) 447 WP_047758064.1 competence type IV pilus minor pilin ComGF -
  RA957_RS12690 (RA957_12690) - 2513292..2513618 (-) 327 WP_047758065.1 type II secretion system protein -
  RA957_RS12695 (RA957_12695) comGD 2513602..2514039 (-) 438 WP_011231914.1 competence type IV pilus minor pilin ComGD -
  RA957_RS12700 (RA957_12700) comGC 2514029..2514325 (-) 297 WP_011231915.1 competence type IV pilus major pilin ComGC Machinery gene
  RA957_RS12705 (RA957_12705) comGB 2514528..2515556 (-) 1029 WP_144329562.1 competence type IV pilus assembly protein ComGB -
  RA957_RS12710 (RA957_12710) comGA 2515553..2516626 (-) 1074 WP_011231917.1 competence type IV pilus ATPase ComGA Machinery gene
  RA957_RS12715 (RA957_12715) - 2516819..2517517 (+) 699 WP_011231918.1 metalloregulator ArsR/SmtB family transcription factor -
  RA957_RS12720 (RA957_12720) - 2517676..2517918 (+) 243 WP_008879854.1 DUF2626 family protein -
  RA957_RS12725 (RA957_12725) - 2518002..2519135 (-) 1134 WP_119878023.1 class I SAM-dependent methyltransferase -
  RA957_RS12730 (RA957_12730) - 2519457..2520089 (-) 633 WP_011231920.1 MBL fold metallo-hydrolase -
  RA957_RS12735 (RA957_12735) - 2520259..2520435 (+) 177 WP_011231921.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39570.00 Da        Isoelectric Point: 8.7901

>NTDB_id=871620 RA957_RS12710 WP_011231917.1 2515553..2516626(-) (comGA) [Geobacillus kaustophilus strain T-1]
MLNEIEQTANRLLAEAVQRRASDLHLVPRRRDAAVRLRLDGMLVDVGVLSKETAERLIAHFKFLAGMDIGERRRPQSGAM
EVAEFGETVYLRLSTLPTLYDESLVIRLLPQRFSLPLRELSLFFRSTARLFSFMQHPQGLVLLTGPTGSGKTTTLYTLLD
LCQAERQRNIITLEDPIEKQNERLLQVQINEKAGITYAAGLKAALRHDPDVLMVGEIRDHDTAAIAIRSALSGHLVVSTM
HAADAVGAVYRLHEFGIPLGDLAETLLAVSAQRLVELCCPLCGDDCHPSCGRLGRRRRTAVYELLCGSALEDVIRSLSNG
RGKPKRSYMTLARLIRKGIALGYLPVRMLELVGGGER

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=871620 RA957_RS12710 WP_011231917.1 2515553..2516626(-) (comGA) [Geobacillus kaustophilus strain T-1]
GTGCTGAACGAGATCGAACAAACGGCAAACCGCCTTCTCGCCGAAGCGGTGCAGCGCCGCGCCTCGGATCTTCACCTCGT
TCCGCGCCGCCGCGATGCCGCTGTTCGTCTTCGGCTTGATGGCATGCTTGTCGACGTTGGCGTGCTTTCGAAGGAAACGG
CGGAACGTCTCATCGCCCACTTTAAATTTTTAGCCGGCATGGACATCGGGGAACGACGCCGTCCGCAAAGCGGCGCCATG
GAAGTGGCTGAGTTTGGGGAAACGGTGTATTTGCGTTTATCGACATTGCCGACGCTCTACGATGAAAGCCTCGTCATTCG
GCTTCTGCCGCAGCGCTTCTCGCTGCCGCTTCGCGAACTATCTTTATTCTTCCGCTCCACCGCACGATTATTTTCCTTCA
TGCAGCATCCGCAAGGGCTTGTGCTGTTGACCGGCCCGACGGGGTCGGGAAAAACGACGACCCTCTACACCCTTCTTGAT
CTTTGCCAAGCTGAAAGGCAACGCAACATCATCACGCTTGAAGATCCGATCGAAAAACAGAACGAACGATTGTTGCAAGT
GCAAATCAATGAAAAAGCAGGCATCACATACGCCGCCGGGTTAAAAGCGGCGCTGCGCCATGACCCGGATGTGTTGATGG
TCGGCGAGATCCGCGATCATGATACGGCGGCCATTGCCATCCGTTCAGCGTTGAGCGGCCATTTGGTCGTCTCGACGATG
CACGCCGCCGATGCAGTCGGCGCCGTCTACCGGCTGCATGAATTCGGCATTCCGCTCGGCGATTTGGCCGAGACGCTGCT
TGCCGTCTCTGCCCAGCGGCTTGTCGAGCTTTGCTGCCCGCTGTGCGGCGACGATTGCCATCCATCGTGCGGCCGTCTTG
GACGACGCCGGCGCACGGCTGTTTATGAGTTGCTTTGCGGTTCAGCGCTGGAGGACGTGATTCGTTCCCTCTCCAATGGG
AGAGGGAAGCCAAAGCGCTCTTATATGACGCTGGCGCGCTTGATCCGCAAGGGGATTGCGCTCGGCTACCTGCCCGTTCG
CATGCTTGAGCTCGTCGGAGGGGGAGAAAGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5KX66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

50.725

96.639

0.49