Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RAM32_RS11845 Genome accession   NZ_CP132361
Coordinates   2405827..2406513 (-) Length   228 a.a.
NCBI ID   WP_072353822.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UNC_SA56     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2400827..2411513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM32_RS11825 (RAM32_11815) rpsT 2401741..2401992 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  RAM32_RS11830 (RAM32_11820) holA 2402037..2403011 (-) 975 WP_001282570.1 DNA polymerase III subunit delta -
  RAM32_RS11835 (RAM32_11825) comEC 2403068..2405269 (-) 2202 WP_025175028.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RAM32_RS11840 (RAM32_11830) - 2405274..2405735 (-) 462 WP_000439693.1 ComE operon protein 2 -
  RAM32_RS11845 (RAM32_11835) comEA 2405827..2406513 (-) 687 WP_072353822.1 ComEA family DNA-binding protein Machinery gene
  RAM32_RS11850 (RAM32_11840) - 2406553..2407269 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  RAM32_RS11855 (RAM32_11845) rsfS 2407272..2407625 (-) 354 WP_001088020.1 ribosome silencing factor -
  RAM32_RS11860 (RAM32_11850) yqeK 2407626..2408210 (-) 585 WP_001019320.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RAM32_RS11865 (RAM32_11855) nadD 2408200..2408769 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  RAM32_RS11870 (RAM32_11860) yhbY 2408772..2409062 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  RAM32_RS11875 (RAM32_11865) aroE 2409066..2409872 (-) 807 WP_000666748.1 shikimate dehydrogenase -
  RAM32_RS11880 (RAM32_11870) yqeH 2409886..2410986 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25773.50 Da        Isoelectric Point: 9.6753

>NTDB_id=868461 RAM32_RS11845 WP_072353822.1 2405827..2406513(-) (comEA) [Staphylococcus aureus strain UNC_SA56]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=868461 RAM32_RS11845 WP_072353822.1 2405827..2406513(-) (comEA) [Staphylococcus aureus strain UNC_SA56]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

99.561

100

0.996
  comEA Staphylococcus aureus N315

97.807

100

0.978