Detailed information NTG0866050

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Q7565_RS14240
• Genome accession: NZ_CP131903
• Coordinates: 2953825..2954943 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 2010N17-053
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2948825..2959943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7565_RS14230 (Q7565_14265)	-	2951941..2952633 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
Q7565_RS14235 (Q7565_14270)	pilT	2952760..2953797 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
Q7565_RS14240 (Q7565_14275)	pilU	2953825..2954943 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Q7565_RS14245 (Q7565_14280)	fur	2955013..2955450 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
Q7565_RS14250 (Q7565_14285)	bamE	2955562..2955960 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
Q7565_RS14255 (Q7565_14290)	-	2956015..2956338 (-)	324	WP_000442587.1	RnfH family protein	-
Q7565_RS14260 (Q7565_14295)	-	2956338..2957405 (-)	1068	WP_002019572.1	hypothetical protein	-
Q7565_RS14265 (Q7565_14300)	-	2957558..2958013 (-)	456	WP_000782976.1	bacteriohemerythrin	-
Q7565_RS14270 (Q7565_14305)	-	2958266..2959114 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=866050 Q7565_RS14240 WP_000347039.1 2953825..2954943(+) (pilU) [Acinetobacter baumannii strain 2010N17-053]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=866050 Q7565_RS14240 WP_000347039.1 2953825..2954943(+) (pilU) [Acinetobacter baumannii strain 2010N17-053]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395