Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   N0B18_RS01965 Genome accession   NZ_CP103995
Coordinates   368222..368671 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain SRCM117109     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 363222..373671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B18_RS01925 (N0B18_01925) - 363547..363744 (+) 198 WP_121509395.1 hypothetical protein -
  N0B18_RS01930 (N0B18_01930) yckC 363764..364219 (+) 456 WP_014478793.1 RDD family protein -
  N0B18_RS01935 (N0B18_01935) yckD 364301..364633 (+) 333 WP_014478794.1 YckD family protein -
  N0B18_RS01940 (N0B18_01940) - 364761..365183 (+) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  N0B18_RS01945 (N0B18_01945) - 365171..365491 (+) 321 WP_014478796.1 DUF3147 family protein -
  N0B18_RS01950 (N0B18_01950) - 365510..366254 (+) 745 Protein_341 AAA family ATPase -
  N0B18_RS01955 (N0B18_01955) bglC 366327..367760 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  N0B18_RS01960 (N0B18_01960) nin/comJ 367797..368195 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  N0B18_RS01965 (N0B18_01965) nucA/comI 368222..368671 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  N0B18_RS01970 (N0B18_01970) tlpC 368839..370560 (-) 1722 Protein_345 methyl-accepting chemotaxis protein TlpC -
  N0B18_RS01975 (N0B18_01975) hxlB 370671..371228 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  N0B18_RS01980 (N0B18_01980) hxlA 371234..371866 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  N0B18_RS01985 (N0B18_01985) hxlR 372095..372457 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=726807 N0B18_RS01965 WP_014475770.1 368222..368671(-) (nucA/comI) [Bacillus subtilis strain SRCM117109]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=726807 N0B18_RS01965 WP_014475770.1 368222..368671(-) (nucA/comI) [Bacillus subtilis strain SRCM117109]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987