Detailed information NTG0708943

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: NL113_RS03805
• Genome accession: NZ_CP101130
• Coordinates: 779386..783075 (-)
• Length: 1229 a.a.
• NCBI ID: WP_006636760.1
• Uniprot ID: M5P7R6
• Organism: Bacillus sp. KRF7
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 774386..788075

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NL113_RS03790	-	774441..774659 (+)	219	WP_006636763.1	spore germination protein	-
NL113_RS03795	sbcC	774743..778135 (-)	3393	WP_006636762.1	exonuclease subunit SbcC	-
NL113_RS03800	sbcD	778132..779316 (-)	1185	WP_006636761.1	exonuclease subunit SbcD	-
NL113_RS03805	addA	779386..783075 (-)	3690	WP_006636760.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
NL113_RS03810	addB	783062..786562 (-)	3501	WP_006636759.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
NL113_RS03815	modB	786672..787328 (-)	657	WP_006636758.1	molybdate ABC transporter permease subunit	-

Sequence

Protein

• Length: 1229 a.a.
• Molecular weight: 141359.10 Da
• Isoelectric Point: 5.4615

>NTDB_id=708943 NL113_RS03805 WP_006636760.1 779386..783075(-) (addA) [Bacillus sp. KRF7]
MEMAKPKNSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITAEDNPIDVDRLLVVTFTNASAAEMKHRIGEA
LERELAEHPGSLHLRRQLSLLNKASISTLHSFCLQVVRKYYYMIDVDPAFRIADQTEGELLGDEVLDELFEEEYQKGDPA
FFELVDRYTTDRHDLDLQYLVKRVYEFSRSHPDPESWLEKFVELYDADADAKVEALPFYPYIKEDVELALKGVREKLARA
LELAKQPGGPAPRAENFLDDLAQIDNLIRHQDDFAALYKLVSAVSFQRAKPCKGDEFEPSLIEEATELRNSAKKQLEKLK
TDYFTRTPDQHLASLKEMKPVIKTLVKLVIEFGRRFKAVKKEKAIVDFSDLEHDCLAILTKKNEAGETVPSEAAAHYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGAGQKIDLNKNFRSR
SDILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDSTKTEVLMIENQENSADEEAEELETVQLEARAMAGRIREL
ISGKFQVYDGKTKTTRNIEYRDIVILLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVAVTLSLLKIIDNAYQDIPL
ASVLRSPIVGLDENELSLIRIRDKKAPFYEALKAYIASSDPDDTLNEKLSRFDRLLKKWRTYAKHHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYESTSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFLVG
TAKDKEKLLAKWRSSASRSEWLLPDFERFQAKSYLDFIGPALMRHRDMSDDLPSPNDEIYHHPSRFTISWISSAELEAED
QAHTEEEKHDRLSLIKKGLPIDGPFDYEEEVRERLTWSYPYQDASRIRTKQSVSEIKRQKEYEDEYGDRSLIRPAQETVL
FKRPSFMMAKGLTAAERGTAMHTVMQHLPLTHAPQADELGRLLDRLVEKELLTEDQRAAIEEDDILAFFETDIGEKLLSA
RRVEREVPFNMTLPAQDVYPGMNTADEPVLIQGIIDCLFETDDGFYLLDYKTDRIHGKFRSGFEGAEPILRKRYETQINL
YARAVETMLKTPLKGKALYFFDGCHVLMF

Nucleotide

• Length: 3690 bp

>NTDB_id=708943 NL113_RS03805 WP_006636760.1 779386..783075(-) (addA) [Bacillus sp. KRF7]
ATGGAGATGGCTAAGCCGAAGAACAGCACATGGACGGATGATCAATGGAAAGCGATTGTCTCATCCGGACGGGACATTCT
TGTCGCGGCGGCCGCCGGTTCCGGAAAAACGGCTGTCCTTGTCGAGCGGATCATCCGGAAAATTACGGCCGAGGATAACC
CGATCGATGTCGACCGGCTGCTTGTCGTCACATTTACAAACGCATCCGCAGCCGAAATGAAGCACAGGATAGGTGAAGCG
CTTGAAAGGGAGCTTGCCGAACATCCCGGCTCCCTTCATTTGCGCAGGCAGCTTTCCTTGCTGAACAAGGCAAGCATTTC
AACCTTGCACTCTTTTTGCCTTCAGGTCGTTCGCAAATATTATTATATGATCGACGTTGATCCGGCCTTTCGGATCGCCG
ACCAGACAGAAGGAGAGCTGCTTGGCGATGAAGTGCTTGACGAGCTGTTTGAGGAAGAATATCAAAAAGGCGATCCGGCT
TTTTTTGAACTGGTTGATCGATATACGACAGACCGCCATGATCTGGATTTGCAGTACCTGGTGAAGCGGGTCTATGAGTT
TTCCCGCTCTCATCCCGATCCGGAATCCTGGCTTGAAAAGTTTGTCGAGCTGTATGACGCAGATGCTGACGCGAAGGTAG
AAGCACTGCCGTTTTATCCTTATATCAAGGAAGATGTCGAACTCGCTCTGAAAGGTGTGCGGGAAAAGCTGGCCCGTGCG
CTTGAGCTGGCAAAACAGCCGGGAGGCCCTGCGCCGCGCGCGGAAAACTTTCTCGATGATTTGGCGCAGATCGACAATCT
GATCAGGCATCAGGATGATTTCGCGGCGCTTTATAAGCTTGTGTCCGCCGTTTCATTTCAACGGGCAAAGCCGTGCAAAG
GAGATGAATTCGAGCCGTCTTTAATCGAAGAAGCGACAGAGCTGCGAAACAGTGCGAAAAAACAGCTGGAAAAGCTGAAA
ACCGATTATTTCACGCGGACTCCTGATCAGCATTTGGCAAGTTTGAAGGAGATGAAGCCCGTCATCAAGACGCTTGTCAA
GCTTGTCATCGAATTCGGCAGACGCTTTAAGGCGGTGAAAAAAGAAAAGGCCATCGTCGATTTCTCCGATCTTGAACACG
ACTGTCTGGCGATTTTGACAAAGAAAAATGAAGCGGGGGAAACGGTCCCGAGCGAAGCTGCGGCCCATTACCGGCATCAG
TTCCATGAAGTGCTTGTCGATGAGTACCAGGATACAAACCTTGTCCAGGAGGCCATCTTAAAGCTGGTCGCCAAGGAGGA
GCATGAGGGAAATCTCTTTATGGTGGGCGATGTCAAGCAGTCGATCTACCGATTCCGCCTGGCTGAGCCGCTGTTATTCC
TCTCAAAATATAAACGGTTTACAGATGATGGATCAGGCGCCGGACAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCCGACATTTTAGACAGCACCAACTTTCTGTTTAAGCAGCTTATGGGGGGAAAGGTCGGCGAAGTTGACTATGACGAGCA
GGCAGAATTAAAGCTCGGCGCGTCATATCCTCCAAATGACAGCACAAAAACGGAAGTGCTGATGATTGAAAATCAGGAAA
ACAGCGCGGATGAAGAGGCGGAAGAGCTGGAAACGGTTCAGCTTGAGGCGAGGGCCATGGCAGGACGGATCCGCGAGCTG
ATATCCGGGAAATTCCAAGTGTATGACGGCAAAACGAAAACAACGAGAAACATCGAGTACCGCGACATCGTGATTTTATT
GCGGTCAATGCCGTGGGCGCCGCAGATCATGGATGAATTCAAACAGCAGGGAATTCCGGTTTACGCGAATTTGTCAACAG
GCTATTTTGAAGCGACAGAAGTCGCAGTCACGCTTTCGCTGCTGAAGATAATCGATAATGCATACCAGGATATCCCGCTG
GCATCCGTTCTCAGATCGCCGATTGTCGGCCTTGATGAAAATGAGCTGTCCCTGATCAGAATCAGGGATAAAAAAGCTCC
TTTTTATGAAGCGCTGAAAGCGTATATCGCCTCGTCCGACCCGGATGACACCCTTAATGAAAAGCTGAGCCGCTTTGACC
GGCTCCTCAAAAAGTGGAGGACATATGCAAAACATCATTCAGTGGCTGAGCTGATCTGGGAAATTTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAGCAGCGGCAGGCCAACTTGCGCGCGCTTTACGACAGGGCCAGATC
ATATGAATCGACATCCTTTCGCGGCTTGTTCCGCTTTTTGAGATTTATAGAACGAATGCAGGAGCGGGGCGATGATCTCG
GAACCGCAAGAGCTTTGAGCGAACAGGAAGACGTCGTCCGGCTGATGACGATTCATTCAAGCAAAGGGCTTGAGTTTCCA
GTCGTATTTACGGCAGGACTTGGCCGGAATTTTAACATGATGGACTTAAACAAATCATATCTGCTTGATAAAGAGCTCGG
TTTCGGGACAAAGTTTATCCATCCAAAATGGCGGATCAGTTATCCGACGCTGCCGCTTATCGCAATGAAGAAAAAAATGC
GCCGCGAGCTTTTATCAGAAGAGCTCCGCGTCCTGTATGTCGCCCTAACCCGGGCAAAGGAAAAGCTGTTTTTGGTCGGT
ACGGCGAAGGACAAGGAAAAGCTATTGGCAAAATGGCGGTCTTCCGCTTCCCGATCCGAATGGCTGCTGCCTGATTTTGA
ACGTTTTCAGGCCAAATCCTATCTCGATTTTATCGGGCCCGCATTAATGCGGCACAGGGATATGTCTGACGATTTACCGT
CGCCGAATGATGAAATCTATCATCATCCGTCCCGTTTTACGATCAGCTGGATCTCTTCCGCAGAGCTTGAAGCTGAAGAT
CAGGCTCACACCGAGGAGGAAAAGCATGACCGGCTGTCACTGATTAAAAAAGGGCTGCCGATCGATGGACCATTTGATTA
TGAGGAGGAAGTGCGCGAGAGGCTGACATGGTCATACCCTTATCAAGACGCGTCCCGCATCCGCACAAAGCAGTCTGTCT
CCGAAATCAAAAGGCAGAAGGAATATGAGGATGAATATGGCGACCGATCATTGATTCGCCCGGCACAAGAGACTGTATTG
TTCAAAAGGCCTTCCTTTATGATGGCGAAAGGGCTGACGGCTGCCGAACGGGGAACGGCGATGCATACGGTCATGCAGCA
CCTCCCGTTGACCCATGCCCCGCAAGCGGATGAGCTTGGCCGCCTGCTTGACAGGCTTGTCGAGAAAGAGCTTTTAACAG
AAGATCAGCGTGCGGCGATCGAAGAAGACGATATTCTCGCGTTTTTCGAAACGGACATAGGTGAAAAACTGCTGAGCGCC
CGGCGGGTTGAACGGGAAGTTCCCTTCAATATGACGCTGCCCGCCCAGGACGTATATCCCGGCATGAACACAGCGGATGA
ACCTGTATTGATTCAGGGGATTATTGACTGTTTGTTTGAGACGGATGATGGATTTTATTTGCTCGATTACAAAACAGACC
GGATACACGGCAAATTTAGAAGCGGCTTTGAAGGAGCGGAGCCGATACTGAGAAAACGCTATGAAACACAGATCAATCTC
TATGCGCGGGCCGTCGAAACGATGTTGAAGACGCCTCTGAAAGGAAAGGCCCTTTACTTTTTTGACGGCTGCCATGTGCT
AATGTTTTGA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1050-1221)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

3D structure

• AlphaFold DB: M5P7R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	73.089	100	0.731