Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD69_RS13750 Genome accession   NZ_CP100859
Coordinates   2959370..2960839 (+) Length   489 a.a.
NCBI ID   WP_005281701.1    Uniprot ID   D4F169
Organism   Edwardsiella tarda strain GT21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2954370..2965839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD69_RS13720 (NMD69_13690) - 2954801..2955790 (+) 990 WP_370547894.1 calcium/sodium antiporter -
  NMD69_RS13725 (NMD69_13695) kdsD 2955842..2956828 (+) 987 WP_370547895.1 arabinose-5-phosphate isomerase KdsD -
  NMD69_RS13730 (NMD69_13700) kdsC 2956844..2957410 (+) 567 WP_370547896.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD69_RS13735 (NMD69_13705) lptC 2957407..2957979 (+) 573 WP_005281705.1 LPS export ABC transporter periplasmic protein LptC -
  NMD69_RS13740 (NMD69_13710) lptA 2957954..2958511 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD69_RS13745 (NMD69_13715) lptB 2958518..2959243 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NMD69_RS13750 (NMD69_13720) rpoN/rpoN1 2959370..2960839 (+) 1470 WP_005281701.1 RNA polymerase factor sigma-54 Machinery gene
  NMD69_RS13755 (NMD69_13725) hpf 2960861..2961148 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NMD69_RS13760 (NMD69_13730) ptsN 2961265..2961729 (+) 465 WP_005281697.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD69_RS13765 (NMD69_13735) rapZ 2961837..2962688 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NMD69_RS13770 (NMD69_13740) npr 2962696..2962968 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  NMD69_RS13775 (NMD69_13745) rnk 2963145..2963555 (+) 411 WP_035597411.1 nucleoside diphosphate kinase regulator -
  NMD69_RS13780 (NMD69_13750) - 2963591..2964094 (-) 504 WP_035604652.1 cytochrome b/b6 domain-containing protein -
  NMD69_RS13785 (NMD69_13755) cybC 2964100..2964486 (-) 387 WP_005291336.1 cytochrome b562 -
  NMD69_RS13790 (NMD69_13760) deoD 2964700..2965425 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55075.91 Da        Isoelectric Point: 4.4068

>NTDB_id=707289 NMD69_RS13750 WP_005281701.1 2959370..2960839(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT21]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=707289 NMD69_RS13750 WP_005281701.1 2959370..2960839(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT21]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGTTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGAGGAGATCCTCGAGAGTCTAGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAACACCGGCGAGTCTGACTATGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAATCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGATAGCGACAGCCAGTTCATTCGTAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTGCTGGCAGATATCGCTCAGCAAGTCGAAATGCACGAGTCGACGATCTCACGCGTGACTACGCAGAAG
TTTCTGCACAGTCCGCGCGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACTGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAGCCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4F169

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481