Detailed information NTG0706696

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NLV76_RS13230
• Genome accession: NZ_CP100752
• Coordinates: 2570936..2571553 (-)
• Length: 205 a.a.
• NCBI ID: WP_059293571.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain MEC_B301
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2565936..2576553

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLV76_RS13200 (NLV76_13200)	rpsT	2566240..2566506 (+)	267	WP_010335003.1	30S ribosomal protein S20	-
NLV76_RS13205 (NLV76_13205)	holA	2566521..2567564 (-)	1044	WP_254500991.1	DNA polymerase III subunit delta	-
NLV76_RS13210 (NLV76_13210)	-	2567604..2567753 (-)	150	WP_125825438.1	hypothetical protein	-
NLV76_RS13215 (NLV76_13215)	-	2567794..2567928 (+)	135	WP_010335005.1	YqzM family protein	-
NLV76_RS13220 (NLV76_13220)	comEC	2567967..2570297 (-)	2331	WP_254503640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NLV76_RS13225 (NLV76_13225)	-	2570301..2570870 (-)	570	WP_254500992.1	ComE operon protein 2	-
NLV76_RS13230 (NLV76_13230)	comEA	2570936..2571553 (-)	618	WP_059293571.1	helix-hairpin-helix domain-containing protein	Machinery gene
NLV76_RS13235 (NLV76_13235)	comER	2571637..2572458 (+)	822	WP_254500993.1	late competence protein ComER	-
NLV76_RS13240 (NLV76_13240)	-	2572523..2573266 (-)	744	WP_254500994.1	class I SAM-dependent methyltransferase	-
NLV76_RS13245 (NLV76_13245)	rsfS	2573263..2573619 (-)	357	WP_044154484.1	ribosome silencing factor	-
NLV76_RS13250 (NLV76_13250)	yqeK	2573637..2574197 (-)	561	WP_254500995.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NLV76_RS13255 (NLV76_13255)	-	2574187..2574756 (-)	570	WP_010335014.1	nicotinate-nucleotide adenylyltransferase	-
NLV76_RS13260 (NLV76_13260)	yhbY	2574767..2575057 (-)	291	WP_254500996.1	ribosome assembly RNA-binding protein YhbY	-
NLV76_RS13265 (NLV76_13265)	aroE	2575057..2575893 (-)	837	WP_254500997.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21982.84 Da
• Isoelectric Point: 5.1547

>NTDB_id=706696 NLV76_RS13230 WP_059293571.1 2570936..2571553(-) (comEA) [Bacillus halotolerans strain MEC_B301]
MNWLNQHKTIIMSAGSAAVLIAVLCLFFFSGHNKEPVKQVVSSEKENAEVKQEATKDKAEENIVIDIKGAVKHPGVYEMH
TGDRVSQAIEKAGGIGEKADETQVNLAELLQDGTVVYIPSKGEEAAQQAAGGAVQSSKGKETLVNINTATLEDLQNISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=706696 NLV76_RS13230 WP_059293571.1 2570936..2571553(-) (comEA) [Bacillus halotolerans strain MEC_B301]
TTGAATTGGTTGAACCAACATAAAACGATAATCATGTCAGCGGGCTCTGCTGCTGTTCTCATTGCAGTTCTTTGCCTCTT
TTTTTTCAGCGGACATAACAAAGAGCCTGTAAAACAAGTTGTATCTAGTGAAAAGGAAAATGCTGAGGTCAAGCAGGAAG
CAACAAAAGACAAAGCGGAAGAAAATATTGTAATTGATATAAAAGGCGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
ACGGGAGACAGAGTGTCGCAGGCTATTGAGAAAGCAGGCGGCATTGGTGAAAAAGCAGATGAAACGCAAGTGAATTTGGC
GGAACTTTTACAGGACGGTACAGTGGTGTATATACCGAGTAAGGGAGAAGAGGCGGCACAGCAAGCGGCCGGCGGCGCTG
TACAAAGCAGTAAGGGGAAGGAGACATTGGTCAATATCAATACAGCAACATTAGAGGATTTACAAAACATTTCGGGAGTA
GGGCCTTCAAAAGCCGAAGCGATTATTGCATATCGGGAAGAGAACGGGCGTTTCCAAACAATTGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCTTTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-203)

• SLBB domain (SLBB) (64-119)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	77.67	100	0.78
comEA	Staphylococcus aureus MW2	38.182	100	0.41
comEA	Staphylococcus aureus N315	37.387	100	0.405
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	37.736	100	0.39
comEA	Latilactobacillus sakei subsp. sakei 23K	35.78	100	0.38