Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilV   Type   Machinery gene
Locus tag   M8G39_RS27215 Genome accession   NZ_CP097383
Coordinates   5746602..5747126 (+) Length   174 a.a.
NCBI ID   WP_003116261.1    Uniprot ID   Q7WZP2
Organism   Pseudomonas aeruginosa strain L00-a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5741602..5752126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8G39_RS27195 (M8G39_27195) pilR 5742864..5744201 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  M8G39_RS27200 (M8G39_27200) thiO 5744247..5745341 (-) 1095 WP_003141551.1 glycine oxidase ThiO -
  M8G39_RS27205 (M8G39_27205) - 5745481..5745981 (+) 501 WP_249913945.1 GspH/FimT family pseudopilin -
  M8G39_RS27210 (M8G39_27210) - 5746104..5746592 (+) 489 WP_003141552.1 GspH/FimT family pseudopilin -
  M8G39_RS27215 (M8G39_27215) pilV 5746602..5747126 (+) 525 WP_003116261.1 type IV pilus modification protein PilV Machinery gene
  M8G39_RS27220 (M8G39_27220) - 5747135..5747950 (+) 816 WP_016254061.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M8G39_RS27225 (M8G39_27225) - 5747947..5748552 (+) 606 WP_003116263.1 pilus assembly protein -
  M8G39_RS27230 (M8G39_27230) - 5748566..5752042 (+) 3477 WP_023100239.1 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 18763.51 Da        Isoelectric Point: 4.9113

>NTDB_id=689375 M8G39_RS27215 WP_003116261.1 5746602..5747126(+) (pilV) [Pseudomonas aeruginosa strain L00-a]
MSRETGFSMIEVLVALVLISIGVLGMVAMQGRTIQYTQESVQRNAAAMLASDLMEIMRADPDAVLNLRAQLREDSVYYKA
KGSDFPAAPARCAPLPADAKERLGCWAQQASKDLPGASALLNSQFYICRSPTPGTCDNTKGSAIEIQVAWRAMDGACFNA
SDSTLCTYSVRSEL

Nucleotide


Download         Length: 525 bp        

>NTDB_id=689375 M8G39_RS27215 WP_003116261.1 5746602..5747126(+) (pilV) [Pseudomonas aeruginosa strain L00-a]
ATGTCTCGAGAAACGGGTTTCAGCATGATCGAAGTACTGGTTGCTCTGGTGCTGATCAGCATTGGCGTACTGGGCATGGT
TGCCATGCAAGGGCGCACGATCCAGTACACGCAGGAGTCGGTACAACGCAATGCCGCAGCAATGCTTGCTAGCGACCTGA
TGGAAATAATGCGTGCGGACCCAGATGCCGTACTCAATCTACGCGCCCAACTACGCGAAGACTCGGTCTACTACAAGGCC
AAGGGCAGCGACTTTCCCGCAGCCCCAGCGCGCTGCGCGCCATTGCCAGCAGATGCTAAGGAACGTCTCGGCTGCTGGGC
CCAACAGGCCTCGAAAGACTTGCCGGGAGCCTCCGCACTCTTGAATAGCCAATTCTACATTTGTCGCAGCCCAACCCCGG
GTACCTGCGACAACACCAAAGGCTCGGCCATCGAAATCCAGGTTGCCTGGCGAGCCATGGATGGAGCGTGTTTCAACGCC
TCTGACTCCACCTTGTGCACCTACAGCGTCCGCTCCGAATTGTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7WZP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilV Pseudomonas aeruginosa PAK

59.429

100

0.598