Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M1S28_RS14380 Genome accession   NZ_CP096749
Coordinates   2952579..2953697 (+) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain 5666     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2919167..2963973 2952579..2953697 within 0


Gene organization within MGE regions


Location: 2919167..2963973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1S28_RS14165 (M1S28_14165) - 2919540..2919926 (-) 387 WP_002042962.1 hypothetical protein -
  M1S28_RS14170 (M1S28_14170) - 2919932..2920492 (-) 561 WP_000410652.1 hypothetical protein -
  M1S28_RS14175 (M1S28_14175) - 2920492..2921133 (-) 642 WP_000218438.1 hypothetical protein -
  M1S28_RS14180 (M1S28_14180) - 2921130..2921585 (-) 456 WP_000575723.1 hypothetical protein -
  M1S28_RS14185 (M1S28_14185) - 2921582..2921767 (-) 186 WP_000200038.1 hypothetical protein -
  M1S28_RS14190 (M1S28_14190) - 2921764..2921964 (-) 201 WP_000290738.1 hypothetical protein -
  M1S28_RS14195 (M1S28_14195) - 2921999..2922373 (-) 375 WP_000818520.1 hypothetical protein -
  M1S28_RS14200 (M1S28_14200) - 2922406..2922639 (-) 234 WP_000335917.1 helix-turn-helix transcriptional regulator -
  M1S28_RS14205 (M1S28_14205) - 2922776..2923474 (+) 699 WP_000660939.1 XRE family transcriptional regulator -
  M1S28_RS14210 (M1S28_14210) - 2923485..2923772 (+) 288 WP_000440996.1 hypothetical protein -
  M1S28_RS14215 (M1S28_14215) - 2923984..2924316 (+) 333 WP_000105905.1 hypothetical protein -
  M1S28_RS14220 (M1S28_14220) - 2924357..2925160 (+) 804 WP_033918526.1 DUF2303 family protein -
  M1S28_RS14225 (M1S28_14225) - 2925246..2925506 (+) 261 WP_000350171.1 hypothetical protein -
  M1S28_RS14230 (M1S28_14230) - 2925565..2925894 (+) 330 WP_001072368.1 hypothetical protein -
  M1S28_RS14235 (M1S28_14235) - 2925891..2926262 (+) 372 WP_001260065.1 hypothetical protein -
  M1S28_RS14240 (M1S28_14240) - 2926433..2927113 (+) 681 WP_000643997.1 DNA polymerase III subunit epsilon -
  M1S28_RS14245 (M1S28_14245) - 2927126..2928154 (+) 1029 WP_000805204.1 ead/Ea22-like family protein -
  M1S28_RS14250 (M1S28_14250) - 2928295..2928585 (+) 291 WP_000986115.1 hypothetical protein -
  M1S28_RS14255 (M1S28_14255) - 2928586..2928765 (+) 180 WP_000464381.1 hypothetical protein -
  M1S28_RS14260 (M1S28_14260) - 2928758..2929294 (+) 537 WP_000609004.1 hypothetical protein -
  M1S28_RS14265 (M1S28_14265) - 2929291..2929800 (+) 510 WP_000578497.1 phage protein -
  M1S28_RS14270 (M1S28_14270) - 2929797..2930006 (+) 210 WP_000028957.1 hypothetical protein -
  M1S28_RS14275 (M1S28_14275) - 2930003..2930218 (+) 216 WP_001292077.1 hypothetical protein -
  M1S28_RS14280 (M1S28_14280) - 2930219..2930389 (+) 171 WP_000119264.1 hypothetical protein -
  M1S28_RS14285 (M1S28_14285) - 2930396..2931388 (-) 993 WP_000527288.1 FRG domain-containing protein -
  M1S28_RS14290 (M1S28_14290) - 2931503..2931958 (-) 456 WP_000549863.1 hypothetical protein -
  M1S28_RS14295 (M1S28_14295) - 2931949..2932833 (-) 885 WP_001076477.1 endonuclease/exonuclease/phosphatase family protein -
  M1S28_RS14300 (M1S28_14300) - 2932830..2933987 (-) 1158 WP_000947475.1 site-specific integrase -
  M1S28_RS14310 (M1S28_14310) ggt 2934685..2936670 (-) 1986 WP_001042923.1 gamma-glutamyltransferase -
  M1S28_RS14315 (M1S28_14315) - 2936973..2938496 (-) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  M1S28_RS14320 (M1S28_14320) - 2938503..2939654 (-) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  M1S28_RS14325 (M1S28_14325) - 2939924..2940421 (-) 498 WP_002019577.1 thioesterase family protein -
  M1S28_RS14330 (M1S28_14330) hemB 2940564..2941577 (+) 1014 WP_000222569.1 porphobilinogen synthase -
  M1S28_RS14335 (M1S28_14335) - 2941603..2942718 (+) 1116 WP_000546701.1 FAD-dependent oxidoreductase -
  M1S28_RS14340 (M1S28_14340) - 2942710..2943615 (-) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  M1S28_RS14345 (M1S28_14345) - 2943705..2944157 (-) 453 WP_000164153.1 ABZJ_00895 family protein -
  M1S28_RS14350 (M1S28_14350) - 2944537..2944914 (+) 378 WP_001059438.1 VOC family protein -
  M1S28_RS14355 (M1S28_14355) - 2944982..2945455 (-) 474 WP_000974358.1 OsmC family protein -
  M1S28_RS14360 (M1S28_14360) - 2945619..2946887 (+) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  M1S28_RS14365 (M1S28_14365) - 2946897..2950493 (+) 3597 WP_000698785.1 SbcC/MukB-like Walker B domain-containing protein -
  M1S28_RS14370 (M1S28_14370) - 2950695..2951387 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  M1S28_RS14375 (M1S28_14375) pilT 2951514..2952551 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  M1S28_RS14380 (M1S28_14380) pilU 2952579..2953697 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M1S28_RS14385 (M1S28_14385) fur 2953767..2954204 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  M1S28_RS14390 (M1S28_14390) bamE 2954316..2954714 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  M1S28_RS14395 (M1S28_14395) - 2954769..2955092 (-) 324 WP_000442587.1 RnfH family protein -
  M1S28_RS14400 (M1S28_14400) - 2955092..2956159 (-) 1068 WP_002019572.1 hypothetical protein -
  M1S28_RS14405 (M1S28_14405) - 2956312..2956767 (-) 456 WP_000782976.1 bacteriohemerythrin -
  M1S28_RS14410 (M1S28_14410) - 2957020..2957868 (-) 849 WP_000336548.1 class II glutamine amidotransferase -
  M1S28_RS14415 (M1S28_14415) - 2957977..2958759 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  M1S28_RS14420 (M1S28_14420) argB 2958880..2959788 (-) 909 WP_001135419.1 acetylglutamate kinase -
  M1S28_RS14425 (M1S28_14425) - 2959803..2961221 (-) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  M1S28_RS14430 (M1S28_14430) dut 2961421..2961873 (-) 453 WP_000868152.1 dUTP diphosphatase -
  M1S28_RS14435 (M1S28_14435) rep 2961898..2963937 (-) 2040 WP_000093035.1 DNA helicase Rep -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=683739 M1S28_RS14380 WP_000347039.1 2952579..2953697(+) (pilU) [Acinetobacter baumannii strain 5666]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=683739 M1S28_RS14380 WP_000347039.1 2952579..2953697(+) (pilU) [Acinetobacter baumannii strain 5666]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395