Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MRP56_RS02075 Genome accession   NZ_CP094267
Coordinates   449252..450124 (-) Length   290 a.a.
NCBI ID   WP_065475375.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain A008N2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444252..455124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRP56_RS02050 - 445988..447172 (+) 1185 WP_073351955.1 tetratricopeptide repeat protein -
  MRP56_RS02055 mutT 447181..447594 (+) 414 WP_174892455.1 8-oxo-dGTP diphosphatase MutT -
  MRP56_RS02060 yacG 447655..447849 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  MRP56_RS02065 zapD 447859..448581 (-) 723 WP_043160635.1 cell division protein ZapD -
  MRP56_RS02070 coaE 448619..449233 (-) 615 WP_073351951.1 dephospho-CoA kinase -
  MRP56_RS02075 pilD 449252..450124 (-) 873 WP_065475375.1 A24 family peptidase Machinery gene
  MRP56_RS02080 pilC 450147..451388 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  MRP56_RS02085 pilB 451504..453210 (-) 1707 WP_073351950.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  MRP56_RS02090 tapA 453214..453657 (-) 444 WP_073352603.1 type IVa pilus major pilin TapA -
  MRP56_RS02095 nadC 453980..454837 (-) 858 WP_073351948.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32484.77 Da        Isoelectric Point: 6.7410

>NTDB_id=668480 MRP56_RS02075 WP_065475375.1 449252..450124(-) (pilD) [Aeromonas hydrophila strain A008N2]
MLLITDVFHSLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYLGYFNPETQPQQEERYNLMVPRSACPHCGHA
ITAMENIPLLSWLWLKGRCRECQAPISARYPLVELLTALLSLVVAATFAPGWGLLAALLLTWVLVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLTGGFAPLADAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL
VGAFIGIGLILLRNHHQNKPIPFGPYLAIAGWIALLWGDTITRWYLTTFL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=668480 MRP56_RS02075 WP_065475375.1 449252..450124(-) (pilD) [Aeromonas hydrophila strain A008N2]
ATGTTGCTAATCACTGACGTTTTTCACAGCCTGCCCTGGCTCTATTTCTCGCTGGTATTCCTCTTTTCTCTGATGATCGG
CAGTTTTCTCAACGTGGTCATCCACCGCCTGCCCATCATGCTGGAGCGGGAGTGGCAGGCCGAATATCTCGGCTATTTCA
ATCCAGAGACACAGCCGCAGCAGGAAGAGCGCTACAACCTGATGGTGCCGCGTTCTGCCTGCCCTCATTGCGGCCACGCC
ATCACGGCCATGGAGAACATCCCCCTGCTCAGCTGGCTCTGGCTCAAGGGGCGCTGCCGTGAATGCCAGGCCCCCATCTC
GGCCCGTTATCCTCTGGTGGAATTGCTGACGGCCCTGCTCTCGCTGGTCGTCGCCGCAACATTCGCACCGGGCTGGGGCT
TGCTGGCCGCCCTGTTGCTCACCTGGGTACTGGTGGCGCTCACTTTCATCGATCTGGACAAGATGCTGCTGCCGGATCAG
CTGACCCTGCCACTGCTGTGGGGTGGCCTGTTGTTCAATCTGACAGGGGGATTTGCGCCGCTGGCCGATGCGGTGATCGG
CGCCATGGCCGGCTATCTGGTGCTCTGGAGCCTCTACTGGGCCTTCAAGTTGCTGACCGGCAAGGAAGGGATGGGCTATG
GCGACTTCAAGCTGCTGGCGGCGCTGGGTGCCTGGCTCGGCTGGCAGGCACTGCCCATCGTGTTGCTGCTCTCGTCGCTG
GTTGGTGCTTTCATCGGAATTGGCCTCATTCTGCTGCGCAACCATCATCAGAATAAACCCATCCCCTTCGGCCCCTATCT
GGCCATCGCCGGCTGGATCGCGCTGCTGTGGGGCGATACCATCACCCGCTGGTACCTCACGACGTTTCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.324

98.966

0.607
  pilD Vibrio campbellii strain DS40M4

55.749

98.966

0.552
  pilD Acinetobacter baumannii D1279779

54.225

97.931

0.531
  pilD Acinetobacter nosocomialis M2

54.225

97.931

0.531
  pilD Neisseria gonorrhoeae MS11

48.718

94.138

0.459

Multiple sequence alignment