Detailed information NTG0665945

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MO978_RS09015
• Genome accession: NZ_CP093959
• Coordinates: 1813987..1814634 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis strain FAM 17919
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1808987..1819634

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MO978_RS08990 (MO978_08985)	atpF	1809057..1809563 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
MO978_RS08995 (MO978_08990)	atpB	1809578..1810291 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
MO978_RS09000 (MO978_08995)	-	1810336..1810551 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
MO978_RS09005 (MO978_09000)	-	1810735..1811511 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
MO978_RS09010 (MO978_09005)	comEC	1811796..1814006 (-)	2211	WP_289421744.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MO978_RS09015 (MO978_09010)	comEA	1813987..1814634 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
MO978_RS09020 (MO978_09015)	-	1814694..1816055 (-)	1362	WP_021214597.1	ABC transporter permease	-
MO978_RS09025 (MO978_09020)	-	1816052..1816984 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
MO978_RS09030 (MO978_09025)	-	1817089..1817487 (-)	399	WP_004255236.1	hypothetical protein	-
MO978_RS09035 (MO978_09030)	-	1817591..1818154 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
MO978_RS09040 (MO978_09035)	-	1818327..1819505 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=665945 MO978_RS09015 WP_004255240.1 1813987..1814634(-) (comEA) [Lactococcus lactis strain FAM 17919]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=665945 MO978_RS09015 WP_004255240.1 1813987..1814634(-) (comEA) [Lactococcus lactis strain FAM 17919]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGAGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367