Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LL023_RS04220 Genome accession   NZ_CP086099
Coordinates   884263..885696 (-) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain 197     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 879263..890696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL023_RS04190 (LL023_04195) elbB 880796..881449 (+) 654 WP_032670144.1 isoprenoid biosynthesis glyoxalase ElbB -
  LL023_RS04195 (LL023_04200) mtgA 881446..882171 (+) 726 WP_014171821.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LL023_RS04200 (LL023_04205) npr 882210..882482 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  LL023_RS04205 (LL023_04210) rapZ 882479..883333 (-) 855 WP_014171820.1 RNase adapter RapZ -
  LL023_RS04210 (LL023_04215) ptsN 883379..883870 (-) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LL023_RS04215 (LL023_04220) hpf 883953..884240 (-) 288 WP_228176393.1 ribosome hibernation promoting factor -
  LL023_RS04220 (LL023_04225) rpoN/rpoN1 884263..885696 (-) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  LL023_RS04225 (LL023_04230) lptB 885744..886469 (-) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  LL023_RS04230 (LL023_04235) lptA 886476..887030 (-) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LL023_RS04235 (LL023_04240) lptC 886999..887574 (-) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  LL023_RS04240 (LL023_04245) kdsC 887571..888137 (-) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LL023_RS04245 (LL023_04250) kdsD 888152..889138 (-) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  LL023_RS04250 (LL023_04255) - 889152..890129 (-) 978 WP_020883528.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=622467 LL023_RS04220 WP_014171817.1 884263..885696(-) (rpoN/rpoN1) [Enterobacter ludwigii strain 197]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=622467 LL023_RS04220 WP_014171817.1 884263..885696(-) (rpoN/rpoN1) [Enterobacter ludwigii strain 197]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCTTTCT
CCGATACCGACCGCGCGATTGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAATCACGATTTCCGCAGCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486