Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KU540_RS08645 Genome accession   NZ_CP077894
Coordinates   1707185..1708159 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain 329     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1702185..1713159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU540_RS08610 (KU540_08560) rpmG 1702645..1702794 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  KU540_RS08615 (KU540_08565) - 1703003..1703542 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  KU540_RS08620 (KU540_08570) - 1703554..1705017 (+) 1464 WP_001017870.1 rhomboid family protein -
  KU540_RS08625 (KU540_08575) - 1704998..1705201 (+) 204 WP_000087561.1 YqgQ family protein -
  KU540_RS08630 (KU540_08580) - 1705198..1706184 (+) 987 WP_000161307.1 ROK family glucokinase -
  KU540_RS08635 (KU540_08585) - 1706184..1706513 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KU540_RS08640 (KU540_08590) - 1706510..1707133 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  KU540_RS08645 (KU540_08595) comGA 1707185..1708159 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU540_RS08650 (KU540_08600) comGB 1708131..1709201 (+) 1071 WP_000776414.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU540_RS08655 (KU540_08605) comGC 1709215..1709526 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KU540_RS08660 (KU540_08610) comGD 1709504..1709950 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU540_RS08665 (KU540_08615) comGE 1709937..1710236 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU540_RS08670 (KU540_08620) comGF 1710154..1710651 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU540_RS08675 (KU540_08625) - 1710748..1710894 (+) 147 WP_001789879.1 hypothetical protein -
  KU540_RS08680 (KU540_08630) - 1710884..1711408 (+) 525 WP_001015120.1 shikimate kinase -
  KU540_RS08685 (KU540_08635) gcvT 1711567..1712658 (+) 1092 WP_000093340.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=584698 KU540_RS08645 WP_000697220.1 1707185..1708159(+) (comGA) [Staphylococcus aureus strain 329]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=584698 KU540_RS08645 WP_000697220.1 1707185..1708159(+) (comGA) [Staphylococcus aureus strain 329]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1