Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KO461_RS04300 Genome accession   NZ_CP076449
Coordinates   828806..829258 (-) Length   150 a.a.
NCBI ID   WP_005550263.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain CU1000N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 823806..834258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KO461_RS04270 - 823966..824142 (-) 177 WP_228414662.1 hypothetical protein -
  KO461_RS04275 - 824151..824729 (-) 579 WP_005566183.1 DUF4123 domain-containing protein -
  KO461_RS04280 - 824733..825299 (-) 567 WP_228414663.1 hypothetical protein -
  KO461_RS04285 (KO461_04295) - 825468..826157 (-) 690 WP_005594882.1 prepilin peptidase -
  KO461_RS04290 (KO461_04300) pilC 826154..827377 (-) 1224 WP_005540455.1 type II secretion system F family protein Machinery gene
  KO461_RS04295 (KO461_04305) - 827370..828779 (-) 1410 Protein_845 GspE/PulE family protein -
  KO461_RS04300 (KO461_04310) pilA 828806..829258 (-) 453 WP_005550263.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KO461_RS04305 (KO461_04315) ampD 829380..829934 (+) 555 WP_005540460.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KO461_RS04310 (KO461_04320) nanQ 830408..830878 (-) 471 WP_005540462.1 N-acetylneuraminate anomerase -
  KO461_RS04315 (KO461_04325) pnp 831172..833301 (+) 2130 WP_032997160.1 polyribonucleotide nucleotidyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15503.95 Da        Isoelectric Point: 9.4349

>NTDB_id=575427 KO461_RS04300 WP_005550263.1 828806..829258(-) (pilA) [Aggregatibacter actinomycetemcomitans strain CU1000N]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAATGVSWNAACGNNAGIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=575427 KO461_RS04300 WP_005550263.1 828806..829258(-) (pilA) [Aggregatibacter actinomycetemcomitans strain CU1000N]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCGGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCACCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGGCATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.043

92

0.58
  pilA Haemophilus influenzae Rd KW20

59.028

96

0.567
  pilA Glaesserella parasuis strain SC1401

53.846

95.333

0.513