Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J6320_RS21980 Genome accession   NZ_CP072782
Coordinates   2984265..2984858 (-) Length   197 a.a.
NCBI ID   WP_064354357.1    Uniprot ID   A0AAW7A2J1
Organism   Vibrio alginolyticus strain HY9901     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2979265..2989858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6320_RS21960 (J6320_21845) aroB 2980150..2981250 (-) 1101 WP_248487093.1 3-dehydroquinate synthase -
  J6320_RS21965 (J6320_21850) aroK 2981283..2981801 (-) 519 WP_005381319.1 shikimate kinase AroK -
  J6320_RS21970 (J6320_21855) pilQ 2981989..2983734 (-) 1746 WP_053307551.1 type IV pilus secretin PilQ family protein Machinery gene
  J6320_RS21975 (J6320_21860) pilP 2983760..2984275 (-) 516 WP_151514393.1 pilus assembly protein PilP Machinery gene
  J6320_RS21980 (J6320_21865) pilO 2984265..2984858 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  J6320_RS21985 (J6320_21870) pilN 2984851..2985426 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  J6320_RS21990 (J6320_21875) pilM 2985410..2986426 (-) 1017 WP_017821733.1 type IV pilus assembly protein PilM Machinery gene
  J6320_RS21995 (J6320_21880) - 2986571..2989132 (+) 2562 WP_005381325.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23029.56 Da        Isoelectric Point: 5.0885

>NTDB_id=555643 J6320_RS21980 WP_064354357.1 2984265..2984858(-) (pilO) [Vibrio alginolyticus strain HY9901]
MVDWQDLEIDEMAEWPLLPQIIVIALLIMVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=555643 J6320_RS21980 WP_064354357.1 2984265..2984858(-) (pilO) [Vibrio alginolyticus strain HY9901]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTATAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGTTACGAGTTATTGCTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGTGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACTCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

86.842

96.447

0.838
  pilO Vibrio cholerae strain A1552

70.157

96.954

0.68