Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   J3B47_RS15895 Genome accession   NZ_CP071749
Coordinates   3601469..3602977 (+) Length   502 a.a.
NCBI ID   WP_207865819.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain VBM21885     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3596469..3607977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J3B47_RS15870 (J3B47_15870) kdsD 3597701..3598684 (+) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -
  J3B47_RS15875 (J3B47_15875) - 3598684..3599223 (+) 540 WP_004200146.1 HAD family hydrolase -
  J3B47_RS15880 (J3B47_15880) lptC 3599229..3599843 (+) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  J3B47_RS15885 (J3B47_15885) lptA 3599862..3600527 (+) 666 WP_009936242.1 lipopolysaccharide transport periplasmic protein LptA -
  J3B47_RS15890 (J3B47_15890) lptB 3600524..3601300 (+) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  J3B47_RS15895 (J3B47_15895) rpoN/rpoN1 3601469..3602977 (+) 1509 WP_207865819.1 RNA polymerase factor sigma-54 Machinery gene
  J3B47_RS15900 (J3B47_15900) raiA 3603127..3603486 (+) 360 WP_004195219.1 ribosome-associated translation inhibitor RaiA -
  J3B47_RS15905 (J3B47_15905) ptsN 3603719..3604228 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  J3B47_RS15910 (J3B47_15910) hprK 3604357..3605325 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  J3B47_RS15915 (J3B47_15915) rapZ 3605411..3606304 (+) 894 WP_004195227.1 RNase adapter RapZ -
  J3B47_RS15920 (J3B47_15920) - 3606331..3606963 (+) 633 WP_151270666.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54932.07 Da        Isoelectric Point: 4.9355

>NTDB_id=547445 J3B47_RS15895 WP_207865819.1 3601469..3602977(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain VBM21885]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVRADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=547445 J3B47_RS15895 WP_207865819.1 3601469..3602977(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain VBM21885]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCGCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.689