NTDB

Overview

Name	comYH	Type	Machinery gene
Locus tag	J0J32_RS08535	Genome accession	NZ_CP071285
Coordinates	1737909..1738844 (-)	Length	311 a.a.
NCBI ID	WP_003133316.1	Uniprot ID	A0A1I4GTA7
Organism	Lactococcus garvieae strain LG728
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1732909..1743844

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J0J32_RS08510 (J0J32_08510)	-	1732957..1733817 (-)	861	WP_003133325.1	S1 RNA-binding domain-containing protein	-
J0J32_RS08515 (J0J32_08515)	frr	1733925..1734482 (-)	558	WP_003133324.1	ribosome recycling factor	-
J0J32_RS08520 (J0J32_08520)	pyrH	1734512..1735228 (-)	717	WP_003133322.1	UMP kinase	-
J0J32_RS08525 (J0J32_08525)	-	1735319..1736512 (-)	1194	WP_003133320.1	acetate kinase	-
J0J32_RS08530 (J0J32_08530)	-	1736631..1737824 (-)	1194	WP_003133318.1	acetate kinase	-
J0J32_RS08535 (J0J32_08535)	comYH	1737909..1738844 (-)	936	WP_003133316.1	class I SAM-dependent methyltransferase	Machinery gene
J0J32_RS08540 (J0J32_08540)	-	1738890..1739120 (-)	231	WP_003133314.1	DUF3165 family protein	-
J0J32_RS08545 (J0J32_08545)	typA	1739195..1741036 (-)	1842	WP_003133313.1	translational GTPase TypA	-
J0J32_RS08550 (J0J32_08550)	-	1741357..1741569 (+)	213	WP_003133311.1	YqgQ family protein	-
J0J32_RS08555 (J0J32_08555)	-	1741562..1742527 (+)	966	WP_003133309.1	ROK family glucokinase	-
J0J32_RS08560 (J0J32_08560)	-	1742547..1742927 (+)	381	WP_003133308.1	rhodanese-like domain-containing protein	-
J0J32_RS08565 (J0J32_08565)	-	1742967..1743413 (-)	447	WP_003133306.1	Dps family protein	-

Sequence

Protein

Download Length: 311 a.a. Molecular weight: 35124.17 Da Isoelectric Point: 4.6693

                                >NTDB_id=544597 J0J32_RS08535 WP_003133316.1 1737909..1738844(-) (comYH) [Lactococcus garvieae strain LG728]

MDIEKIEKAFGLVLENIMAIQAELLTDFYDAFVEQNAAYLGALEFASLTKENNDKVRSMNLTKSEWQKLFQFVLLQGSRV

APMQANHNLTPDSIGFIFNFIIEELHKERKIRVLEFGSGTGNLAETLLVHMQKEVDYVGFEVDDLLLDLSASMSEVMGTE

ASFLQIDAVKPQPLESVDVVMSDLPVGYYPDDDTARHFQVHDKSEHTYVHHLMIEQSFKYLKESGFALFLAPDNLLTSAQ

SEFLKAWIKEHAHVAAVITLPSSLFKGAGKSIYLLSKNQTNTPTFVYPLADLADRSILQEFMSEFVKNVKI

Nucleotide

Download Length: 936 bp

                                >NTDB_id=544597 J0J32_RS08535 WP_003133316.1 1737909..1738844(-) (comYH) [Lactococcus garvieae strain LG728]

ATGGATATAGAGAAAATCGAAAAAGCCTTTGGATTGGTTCTTGAAAATATTATGGCAATTCAAGCGGAGCTCTTGACGGA

TTTTTATGATGCTTTTGTGGAACAAAATGCAGCTTACTTAGGCGCTTTAGAGTTTGCATCTCTGACGAAGGAAAATAATG

ATAAGGTTCGTTCAATGAACCTCACGAAAAGTGAGTGGCAAAAGCTGTTTCAATTTGTCTTGCTTCAAGGGTCACGTGTC

GCACCAATGCAAGCAAATCATAATTTAACACCAGATTCTATTGGGTTTATCTTTAATTTCATCATTGAAGAGTTGCACAA

AGAAAGAAAGATTCGTGTACTAGAATTTGGCTCAGGAACAGGGAATTTAGCGGAAACGCTCTTGGTTCATATGCAAAAAG

AAGTAGACTATGTTGGTTTTGAAGTAGATGACCTCTTGTTGGATTTGTCCGCTTCAATGTCCGAAGTCATGGGAACAGAA

GCAAGTTTTCTCCAAATTGACGCCGTGAAACCACAGCCCTTGGAGTCTGTAGATGTTGTGATGAGTGATCTCCCGGTTGG

TTATTATCCAGACGATGACACTGCTCGCCATTTTCAAGTGCATGACAAATCAGAACATACCTATGTGCACCACTTAATGA

TTGAGCAATCCTTTAAGTATCTGAAGGAGAGTGGTTTTGCGCTCTTCTTAGCTCCAGATAATCTTTTGACAAGTGCACAG

TCAGAATTTTTGAAGGCTTGGATTAAAGAACATGCACATGTTGCTGCAGTCATTACATTGCCTTCATCACTCTTTAAAGG

TGCAGGAAAATCGATTTATTTATTAAGTAAAAACCAAACAAATACACCAACTTTTGTTTACCCCTTGGCTGATTTAGCAG

ACCGTTCTATTTTGCAAGAATTTATGTCTGAGTTTGTGAAAAATGTCAAGATTTGA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(132-286)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A1I4GTA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comYH	Streptococcus mutans UA140	49.677	99.678	0.495
comYH	Streptococcus mutans UA159	49.355	99.678	0.492

Detailed information NTG0544597