Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JYA70_RS02340 Genome accession   NZ_CP071102
Coordinates   434448..435422 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain CI/BAC/25/13/W     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 429448..440422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYA70_RS02305 (JYA70_000461) rpmG 429908..430057 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  JYA70_RS02310 (JYA70_000462) - 430266..430805 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  JYA70_RS02315 (JYA70_000463) - 430817..432280 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  JYA70_RS02320 (JYA70_000464) - 432261..432464 (+) 204 WP_000087561.1 YqgQ family protein -
  JYA70_RS02325 (JYA70_000465) - 432461..433447 (+) 987 WP_000161314.1 ROK family glucokinase -
  JYA70_RS02330 (JYA70_000466) - 433447..433776 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  JYA70_RS02335 (JYA70_000467) - 433773..434396 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  JYA70_RS02340 (JYA70_000468) comGA 434448..435422 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  JYA70_RS02345 (JYA70_000469) comGB 435394..436464 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  JYA70_RS02350 (JYA70_000470) comGC 436478..436789 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  JYA70_RS02355 (JYA70_000471) comGD 436767..437213 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  JYA70_RS02360 (JYA70_000472) comGE 437200..437499 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  JYA70_RS02365 (JYA70_000473) comGF 437417..437914 (+) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  JYA70_RS02370 (JYA70_000474) - 438011..438157 (+) 147 WP_072399793.1 hypothetical protein -
  JYA70_RS02375 (JYA70_000475) - 438147..438671 (+) 525 WP_001015120.1 shikimate kinase -
  JYA70_RS02380 (JYA70_000476) gcvT 438830..439921 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=542845 JYA70_RS02340 WP_000697220.1 434448..435422(+) (comGA) [Staphylococcus aureus strain CI/BAC/25/13/W]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=542845 JYA70_RS02340 WP_000697220.1 434448..435422(+) (comGA) [Staphylococcus aureus strain CI/BAC/25/13/W]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1