Detailed information NTG0534222

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JQ490_RS06970
• Genome accession: NZ_CP069378
• Coordinates: 1432953..1433600 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis strain Ge001
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1427953..1438600

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQ490_RS06945 (JQ490_06945)	atpF	1428023..1428529 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
JQ490_RS06950 (JQ490_06950)	atpB	1428544..1429257 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
JQ490_RS06955 (JQ490_06955)	-	1429302..1429517 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
JQ490_RS06960 (JQ490_06960)	-	1429701..1430477 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
JQ490_RS06965 (JQ490_06965)	comEC	1430762..1432972 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JQ490_RS06970 (JQ490_06970)	comEA	1432953..1433600 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
JQ490_RS06975 (JQ490_06975)	-	1433660..1435021 (-)	1362	WP_021214597.1	ABC transporter permease	-
JQ490_RS06980 (JQ490_06980)	-	1435018..1435950 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
JQ490_RS06985 (JQ490_06985)	-	1436055..1436453 (-)	399	WP_004255236.1	hypothetical protein	-
JQ490_RS06990 (JQ490_06990)	-	1436557..1437120 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
JQ490_RS06995 (JQ490_06995)	-	1437293..1438471 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=534222 JQ490_RS06970 WP_010906131.1 1432953..1433600(-) (comEA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain Ge001]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=534222 JQ490_RS06970 WP_010906131.1 1432953..1433600(-) (comEA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain Ge001]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386