Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   I1A60_RS07850 Genome accession   NZ_CP065857
Coordinates   1628417..1628914 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CC1153-MRSA     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1623417..1633914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I1A60_RS07825 (I1A60_07825) gcvPB 1623579..1625051 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  I1A60_RS07830 (I1A60_07830) gcvPA 1625044..1626390 (-) 1347 WP_043854778.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  I1A60_RS07835 (I1A60_07835) gcvT 1626410..1627501 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  I1A60_RS07840 (I1A60_07840) - 1627660..1628184 (-) 525 WP_001015121.1 shikimate kinase -
  I1A60_RS07845 (I1A60_07845) - 1628174..1628320 (-) 147 WP_001789879.1 hypothetical protein -
  I1A60_RS07850 (I1A60_07850) comGF 1628417..1628914 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  I1A60_RS07855 (I1A60_07855) comGE 1628832..1629131 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  I1A60_RS07860 (I1A60_07860) comGD 1629118..1629564 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  I1A60_RS07865 (I1A60_07865) comGC 1629542..1629853 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  I1A60_RS07870 (I1A60_07870) comGB 1629867..1630937 (-) 1071 WP_031882658.1 competence type IV pilus assembly protein ComGB Machinery gene
  I1A60_RS07875 (I1A60_07875) comGA 1630909..1631883 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  I1A60_RS07880 (I1A60_07880) - 1631935..1632558 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  I1A60_RS07885 (I1A60_07885) - 1632555..1632884 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  I1A60_RS07890 (I1A60_07890) - 1632884..1633870 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=514990 I1A60_RS07850 WP_001789864.1 1628417..1628914(-) (comGF) [Staphylococcus aureus strain CC1153-MRSA]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=514990 I1A60_RS07850 WP_001789864.1 1628417..1628914(-) (comGF) [Staphylococcus aureus strain CC1153-MRSA]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACAGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964