Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IRJ20_RS11555 Genome accession   NZ_CP065137
Coordinates   2282441..2282878 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus sp. A1(2020)     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2277441..2287878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ20_RS11530 - 2278461..2279597 (+) 1137 WP_242750290.1 zinc-dependent alcohol dehydrogenase -
  IRJ20_RS11535 - 2279612..2280046 (+) 435 WP_012116767.1 RDD family protein -
  IRJ20_RS11540 - 2280118..2280441 (+) 324 WP_007409357.1 YckD family protein -
  IRJ20_RS11545 - 2280545..2281981 (+) 1437 WP_098080961.1 family 1 glycosylhydrolase -
  IRJ20_RS11550 nin/comJ 2282022..2282420 (-) 399 WP_039252825.1 competence protein ComJ Regulator
  IRJ20_RS11555 nucA/comI 2282441..2282878 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  IRJ20_RS11560 hxlB 2283236..2283793 (-) 558 WP_039252823.1 6-phospho-3-hexuloisomerase -
  IRJ20_RS11565 hxlA 2283790..2284425 (-) 636 WP_015416785.1 3-hexulose-6-phosphate synthase -
  IRJ20_RS11570 - 2284657..2285019 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=507237 IRJ20_RS11555 WP_003156588.1 2282441..2282878(-) (nucA/comI) [Bacillus sp. A1(2020)]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=507237 IRJ20_RS11555 WP_003156588.1 2282441..2282878(-) (nucA/comI) [Bacillus sp. A1(2020)]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACATCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641