Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I1E95_RS07440 Genome accession   NZ_CP064908
Coordinates   1435000..1436079 (-) Length   359 a.a.
NCBI ID   WP_197166643.1    Uniprot ID   A0A7T1HLN2
Organism   Synechococcus sp. CBW1107     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1430000..1441079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I1E95_RS07420 (I1E95_07410) - 1430595..1431920 (+) 1326 WP_197166642.1 NAD(P)/FAD-dependent oxidoreductase -
  I1E95_RS07425 (I1E95_07415) - 1431885..1432511 (-) 627 Protein_1483 type II secretion system F family protein -
  I1E95_RS07430 (I1E95_07420) - 1432615..1434108 (-) 1494 WP_231594537.1 IS66 family transposase -
  I1E95_RS07435 (I1E95_07425) - 1434192..1434989 (-) 798 Protein_1485 type II secretion system F family protein -
  I1E95_RS07440 (I1E95_07430) pilT 1435000..1436079 (-) 1080 WP_197166643.1 type IV pilus twitching motility protein PilT Machinery gene
  I1E95_RS07445 (I1E95_07435) - 1436136..1437944 (-) 1809 WP_197166644.1 GspE/PulE family protein -
  I1E95_RS07450 (I1E95_07440) grpE 1438042..1438779 (+) 738 WP_197166645.1 nucleotide exchange factor GrpE -
  I1E95_RS07455 (I1E95_07445) dnaJ 1438779..1439894 (+) 1116 WP_197166646.1 molecular chaperone DnaJ -
  I1E95_RS07460 (I1E95_07450) - 1439910..1440203 (+) 294 WP_197166648.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39793.16 Da        Isoelectric Point: 6.0511

>NTDB_id=505598 I1E95_RS07440 WP_197166643.1 1435000..1436079(-) (pilT) [Synechococcus sp. CBW1107]
MELQIEELMEELVEQKGSDLHLSAGQQPYGRFNGELRPMREQALSEESCNRMIFSILTNSQRKQLEQTWELDCSYGLRGL
ARFRINVFSQRGTYAASMRALNSKVPTPQELGLPPVVVDITKRPSGLVLVTGPTGSGKSTTLASLLDHINHSRAEHIITI
EDPIEFAYINDKSLIHQRQLNEDTKSFSNALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPAGQQAQIRTQLSSSLAAIFAQTLCRRSSPSNHNYGRVMAQEIMINTPALANLIREAKTAQIYSQIQTGGQLGM
QTLEKALADMVRQGDITLEEARTHSTKPDELNRLLGNSL

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=505598 I1E95_RS07440 WP_197166643.1 1435000..1436079(-) (pilT) [Synechococcus sp. CBW1107]
ATGGAGCTCCAGATCGAGGAGTTGATGGAAGAGCTGGTCGAGCAGAAGGGCAGCGACCTTCATCTCTCGGCCGGCCAGCA
ACCCTACGGACGCTTCAACGGGGAACTCAGGCCGATGCGGGAGCAGGCACTCAGCGAGGAATCCTGCAACCGCATGATCT
TCTCGATCCTCACCAACTCCCAGCGGAAGCAGCTGGAGCAGACCTGGGAACTCGATTGCTCCTACGGCCTGCGCGGCCTG
GCCCGCTTCCGCATCAATGTCTTCAGTCAGCGCGGCACCTACGCCGCGTCCATGCGGGCCCTGAACAGCAAGGTCCCCAC
TCCCCAGGAGCTCGGCCTGCCTCCGGTGGTGGTGGACATCACCAAGAGGCCTTCGGGACTTGTTCTGGTGACGGGTCCGA
CAGGATCGGGCAAGAGCACCACTCTGGCCTCCCTGCTCGATCACATCAACCACAGCCGTGCCGAGCACATCATCACGATC
GAAGATCCAATCGAGTTTGCTTACATCAATGACAAGAGCCTGATCCATCAGCGCCAGCTCAATGAAGACACCAAAAGCTT
CAGCAATGCTCTGAGAGCAGCGCTGCGTGAGGATCCCGATGTGATTCTGGTGGGGGAAATGCGCGATCTGGAAACGATCC
AGCTGGCCATCACCGCAGCCGAAACCGGCCACCTGGTGTTTGCCACCCTGCACACCAGTTCGGCCGCCCAGACCGTGGAT
CGCATGGTGGATGTGTTCCCGGCGGGCCAGCAGGCCCAGATCCGCACCCAGCTCAGCAGCAGCCTGGCGGCGATCTTCGC
CCAGACACTCTGCCGGAGAAGTTCACCCTCCAACCACAACTATGGCCGGGTGATGGCTCAGGAGATCATGATCAACACAC
CGGCCCTGGCCAATCTGATCCGCGAAGCGAAAACAGCCCAGATCTATTCCCAGATTCAGACCGGCGGACAGCTGGGCATG
CAGACCCTGGAGAAAGCCCTGGCGGACATGGTCAGGCAGGGAGACATCACCCTGGAGGAAGCCCGCACCCACTCCACCAA
GCCGGATGAGCTGAACCGTCTGCTCGGCAACTCCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T1HLN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.436

97.214

0.51
  pilT Acinetobacter baylyi ADP1

51.862

97.214

0.504
  pilT Pseudomonas stutzeri DSM 10701

51.576

97.214

0.501
  pilT Acinetobacter baumannii D1279779

51.289

97.214

0.499
  pilT Acinetobacter nosocomialis M2

51.289

97.214

0.499
  pilT Acinetobacter baumannii strain A118

51.289

97.214

0.499
  pilT Neisseria meningitidis 8013

51.471

94.708

0.487
  pilT Legionella pneumophila strain ERS1305867

49.575

98.329

0.487
  pilT Legionella pneumophila strain Lp02

49.575

98.329

0.487
  pilT Neisseria gonorrhoeae MS11

51.176

94.708

0.485
  pilT Vibrio cholerae strain A1552

50.147

94.986

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.147

94.986

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.011

98.05

0.471
  pilU Pseudomonas stutzeri DSM 10701

37.677

98.329

0.37
  pilU Acinetobacter baylyi ADP1

37.784

98.05

0.37