Detailed information NTG0505089

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLDRC3_RS04155
• Genome accession: NZ_CP064835
• Coordinates: 767114..767761 (+)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain DRC3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 762114..772761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLDRC3_RS04130 (LLDRC3_0783)	-	762243..763421 (+)	1179	WP_021214596.1	SLC13 family permease	-
LLDRC3_RS04135 (LLDRC3_0784)	-	763594..764157 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLDRC3_RS04140 (LLDRC3_0785)	-	764261..764659 (+)	399	WP_004255236.1	hypothetical protein	-
LLDRC3_RS04145 (LLDRC3_0786)	-	764764..765696 (+)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLDRC3_RS04150 (LLDRC3_0787)	-	765693..767054 (+)	1362	WP_021214597.1	ABC transporter permease	-
LLDRC3_RS04155 (LLDRC3_0788)	comEA	767114..767761 (+)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LLDRC3_RS04160 (LLDRC3_0789)	comEC	767742..769880 (+)	2139	WP_302105011.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLDRC3_RS04165 (LLDRC3_0790)	-	770236..771012 (+)	777	WP_063283279.1	alpha/beta hydrolase family protein	-
LLDRC3_RS04170 (LLDRC3_0791)	-	771196..771411 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLDRC3_RS04175 (LLDRC3_0792)	atpB	771456..772169 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLDRC3_RS04180 (LLDRC3_0793)	atpF	772184..772690 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=505089 LLDRC3_RS04155 WP_010906131.1 767114..767761(+) (comEA) [Lactococcus lactis subsp. lactis strain DRC3]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=505089 LLDRC3_RS04155 WP_010906131.1 767114..767761(+) (comEA) [Lactococcus lactis subsp. lactis strain DRC3]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386