Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IF740_RS02380 Genome accession   NZ_CP062344
Coordinates   446108..447082 (+) Length   324 a.a.
NCBI ID   WP_000697235.1    Uniprot ID   A0AB74Q180
Organism   Staphylococcus aureus strain NAS_AN_265     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441108..452082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF740_RS02345 (IF740_02355) rpmG 441567..441716 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  IF740_RS02350 (IF740_02360) - 441925..442464 (+) 540 WP_000164544.1 5-formyltetrahydrofolate cyclo-ligase -
  IF740_RS02355 (IF740_02365) - 442476..443939 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  IF740_RS02360 (IF740_02370) - 443920..444123 (+) 204 WP_000087561.1 YqgQ family protein -
  IF740_RS02365 (IF740_02375) - 444120..445106 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF740_RS02370 (IF740_02380) - 445106..445435 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF740_RS02375 (IF740_02385) - 445432..446055 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF740_RS02380 (IF740_02390) comGA 446108..447082 (+) 975 WP_000697235.1 competence type IV pilus ATPase ComGA Machinery gene
  IF740_RS02385 (IF740_02395) comGB 447054..448124 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF740_RS02390 (IF740_02400) comGC 448138..448449 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF740_RS02395 (IF740_02405) comGD 448427..448873 (+) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF740_RS02400 (IF740_02410) comGE 448860..449159 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF740_RS02405 (IF740_02415) comGF 449077..449574 (+) 498 WP_029694076.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF740_RS02410 (IF740_02420) - 449671..449817 (+) 147 WP_001789879.1 hypothetical protein -
  IF740_RS02415 (IF740_02425) - 449807..450331 (+) 525 WP_001015121.1 shikimate kinase -
  IF740_RS02420 (IF740_02430) gcvT 450490..451581 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36883.93 Da        Isoelectric Point: 8.8516

>NTDB_id=488107 IF740_RS02380 WP_000697235.1 446108..447082(+) (comGA) [Staphylococcus aureus strain NAS_AN_265]
MKILFQEVINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=488107 IF740_RS02380 WP_000697235.1 446108..447082(+) (comGA) [Staphylococcus aureus strain NAS_AN_265]
TTGAAGATTCTATTTCAAGAAGTAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGTAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997