Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HPQ66_RS01800 Genome accession   NZ_CP053764
Coordinates   364699..365136 (-) Length   145 a.a.
NCBI ID   WP_172614434.1    Uniprot ID   -
Organism   Bacillus velezensis strain B268     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359699..370136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPQ66_RS19070 - 360299..360457 (-) 159 Protein_308 hypothetical protein -
  HPQ66_RS01765 (HPQ66_01765) - 360572..361774 (+) 1203 WP_070081342.1 GTP-binding protein -
  HPQ66_RS01770 (HPQ66_01770) - 361839..362975 (+) 1137 WP_052827030.1 zinc-dependent alcohol dehydrogenase -
  HPQ66_RS01775 (HPQ66_01775) - 362990..363424 (+) 435 WP_172614431.1 RDD family protein -
  HPQ66_RS01780 (HPQ66_01780) - 363497..363817 (+) 321 WP_015388719.1 YckD family protein -
  HPQ66_RS01785 (HPQ66_01785) - 363915..364031 (+) 117 WP_172614432.1 family 1 glycosylhydrolase -
  HPQ66_RS01790 (HPQ66_01790) - 364030..364260 (+) 231 Protein_314 family 1 glycosylhydrolase -
  HPQ66_RS01795 (HPQ66_01795) nin/comJ 364279..364677 (-) 399 WP_172614433.1 competence protein ComJ Regulator
  HPQ66_RS01800 (HPQ66_01800) nucA/comI 364699..365136 (-) 438 WP_172614434.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  HPQ66_RS01805 (HPQ66_01805) hxlB 365495..366052 (-) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  HPQ66_RS01810 (HPQ66_01810) hxlA 366049..366684 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  HPQ66_RS01815 (HPQ66_01815) - 366916..367278 (+) 363 WP_016936795.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16071.37 Da        Isoelectric Point: 9.0058

>NTDB_id=446956 HPQ66_RS01800 WP_172614434.1 364699..365136(-) (nucA/comI) [Bacillus velezensis strain B268]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNNPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=446956 HPQ66_RS01800 WP_172614434.1 364699..365136(-) (nucA/comI) [Bacillus velezensis strain B268]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATA
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641