Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HIS92_RS05960 Genome accession   NZ_CP051858
Coordinates   1205314..1205748 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis serovar israelensis strain BGSC 4Q7rifR     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1200314..1210748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIS92_RS05945 (HIS92_05975) - 1201492..1202049 (+) 558 WP_000062064.1 PadR family transcriptional regulator -
  HIS92_RS05950 (HIS92_05980) metE 1202562..1204850 (+) 2289 WP_001007602.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  HIS92_RS05955 (HIS92_05985) - 1204888..1205277 (-) 390 WP_000424022.1 competence protein ComJ -
  HIS92_RS05960 (HIS92_05990) nucA/comI 1205314..1205748 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  HIS92_RS05965 (HIS92_05995) - 1205977..1206726 (+) 750 WP_000388468.1 DUF3967 domain-containing protein -
  HIS92_RS05970 (HIS92_06000) kinB 1206765..1208039 (-) 1275 WP_000420367.1 sporulation sensor histidine kinase KinB -
  HIS92_RS05975 (HIS92_06005) - 1208350..1208550 (+) 201 WP_000929277.1 hypothetical protein -
  HIS92_RS05980 (HIS92_06010) - 1208672..1209835 (+) 1164 WP_000434585.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=440975 HIS92_RS05960 WP_000811494.1 1205314..1205748(-) (nucA/comI) [Bacillus thuringiensis serovar israelensis strain BGSC 4Q7rifR]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=440975 HIS92_RS05960 WP_000811494.1 1205314..1205748(-) (nucA/comI) [Bacillus thuringiensis serovar israelensis strain BGSC 4Q7rifR]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549