Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G8D25_RS10200 Genome accession   NZ_CP051296
Coordinates   501143..502642 (+) Length   499 a.a.
NCBI ID   WP_014618027.1    Uniprot ID   F6G492
Organism   Ralstonia solanacearum strain CIAT_078     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496143..507642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8D25_RS10175 (G8D25_10170) - 497321..498304 (+) 984 WP_014618022.1 KpsF/GutQ family sugar-phosphate isomerase -
  G8D25_RS10180 (G8D25_10175) - 498337..498924 (+) 588 WP_014618023.1 KdsC family phosphatase -
  G8D25_RS10185 (G8D25_10180) lptC 498926..499558 (+) 633 WP_014618024.1 LPS export ABC transporter periplasmic protein LptC -
  G8D25_RS10190 (G8D25_10185) lptA 499626..500223 (+) 598 Protein_490 lipopolysaccharide transport periplasmic protein LptA -
  G8D25_RS10195 (G8D25_10190) lptB 500257..501045 (+) 789 WP_014618026.1 LPS export ABC transporter ATP-binding protein -
  G8D25_RS10200 (G8D25_10195) rpoN/rpoN1 501143..502642 (+) 1500 WP_014618027.1 RNA polymerase factor sigma-54 Machinery gene
  G8D25_RS10205 (G8D25_10200) hpf 502694..503047 (+) 354 WP_003262275.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  G8D25_RS10210 (G8D25_10205) ptsN 503397..503852 (+) 456 WP_003262274.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G8D25_RS10215 (G8D25_10210) hprK 503923..504897 (+) 975 WP_003262273.1 HPr(Ser) kinase/phosphatase -
  G8D25_RS10220 (G8D25_10215) - 505088..505420 (+) 333 WP_039550978.1 PsiF family protein -
  G8D25_RS10225 (G8D25_10220) rapZ 505514..506404 (+) 891 WP_014618030.1 RNase adapter RapZ -
  G8D25_RS10230 (G8D25_10225) - 506482..507135 (+) 654 WP_014618031.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55158.56 Da        Isoelectric Point: 4.8522

>NTDB_id=437290 G8D25_RS10200 WP_014618027.1 501143..502642(+) (rpoN/rpoN1) [Ralstonia solanacearum strain CIAT_078]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPPPTE
SPPPAANGSDHADTAEASYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSMR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLESPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=437290 G8D25_RS10200 WP_014618027.1 501143..502642(+) (rpoN/rpoN1) [Ralstonia solanacearum strain CIAT_078]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGATGGTTCGGTCAGCGCGCTGCAGAGCAACAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCTCGGACCACGCCGACACAGCCGAAGCATCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCCCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAGCCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAATTGTCCATGCGT
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGACGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGTGCACGCTCGGCTGCGGAATGCCTGACGCTGCAGTTGCAGCGCCTGGAGTCGCCAGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAGCTGCTCGCCGCGCGCGATTACACGCGCCTGAAGAAAGCGCTGTCCGTCGACGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCGCCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCGAACCTGCAGCAAAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6G492

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.595

100

0.976