Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HCU79_RS19295 Genome accession   NZ_CP050794
Coordinates   4211860..4213299 (-) Length   479 a.a.
NCBI ID   WP_139438911.1    Uniprot ID   -
Organism   Aeromonas media strain FiBL-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4206860..4218299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU79_RS19265 (HCU79_19265) - 4207133..4207873 (+) 741 WP_404837277.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  HCU79_RS19270 (HCU79_19270) - 4207980..4209884 (+) 1905 WP_404837278.1 BglG family transcription antiterminator -
  HCU79_RS19275 (HCU79_19275) npr 4209903..4210175 (-) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  HCU79_RS19280 (HCU79_19280) rapZ 4210196..4211062 (-) 867 WP_404837279.1 RNase adapter RapZ -
  HCU79_RS19285 (HCU79_19285) ptsN 4211103..4211549 (-) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HCU79_RS19290 (HCU79_19290) hpf 4211552..4211839 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  HCU79_RS19295 (HCU79_19295) rpoN/rpoN1 4211860..4213299 (-) 1440 WP_139438911.1 RNA polymerase factor sigma-54 Machinery gene
  HCU79_RS19300 (HCU79_19300) lptB 4213365..4214090 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  HCU79_RS19305 (HCU79_19305) lptA 4214103..4214621 (-) 519 WP_025328531.1 lipopolysaccharide transport periplasmic protein LptA -
  HCU79_RS19310 (HCU79_19310) lptC 4214608..4215168 (-) 561 WP_139438913.1 LPS export ABC transporter periplasmic protein LptC -
  HCU79_RS19315 (HCU79_19315) kdsC 4215165..4215719 (-) 555 WP_404837280.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HCU79_RS19320 (HCU79_19320) - 4215719..4216714 (-) 996 WP_139438917.1 KpsF/GutQ family sugar-phosphate isomerase -
  HCU79_RS19325 (HCU79_19325) mlaF 4216921..4217721 (+) 801 WP_139438919.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53723.61 Da        Isoelectric Point: 4.3903

>NTDB_id=434908 HCU79_RS19295 WP_139438911.1 4211860..4213299(-) (rpoN/rpoN1) [Aeromonas media strain FiBL-01]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSPIEPQDASQLDSSDAMAQDSMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTASLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGTKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=434908 HCU79_RS19295 WP_139438911.1 4211860..4213299(-) (rpoN/rpoN1) [Aeromonas media strain FiBL-01]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCAGGAAACCAGCCCGATAGAGCCCCAGGACGCCAGTCAACTCGACTCCAGCGACGCCATGGCGCAGGACAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGATGAGGTCTACTCCGCCGGGACTGCCCAGAGCGCCGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAACCTGCAGGACTATCTGCTGTGGCAGATGCGACTGACGCCGTTCA
GCGATCTGGATGCCGCCATCGCCCTCGCCATCATCGATGCCATCGACGAGTCCGGCTACCTGACCGCCTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATAGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGTCAGTACTCGCCGGACCTGCCCT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACCTGCTCGGCAACCGCGACTACCGCACCCTGGCGCGCAAG
ACCCGGCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAATGGCATCAT
CCAGAGCGAGTCGCAATACGTGATCCCGGACGTCTCCGTGGTCAAGAAGGGGACGAAATGGGTGGTTGAGCTCAACCCGG
ATTCGGCGCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCACGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGCTCCCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAATACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.471

100

0.495