Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HBB04_RS09145 Genome accession   NZ_CP050260
Coordinates   2045423..2047117 (+) Length   564 a.a.
NCBI ID   WP_005895655.1    Uniprot ID   A0AAE6QIZ1
Organism   Pseudomonas coronafaciens strain X-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2040423..2052117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBB04_RS09120 (HBB04_01851) - 2041551..2042549 (-) 999 WP_191867870.1 ATP-grasp domain-containing protein -
  HBB04_RS09125 (HBB04_01852) - 2042669..2043214 (+) 546 WP_122354618.1 DUF1523 family protein -
  HBB04_RS09130 (HBB04_01853) - 2043258..2043665 (-) 408 WP_122354616.1 hypothetical protein -
  HBB04_RS09135 (HBB04_01854) - 2043662..2044249 (-) 588 WP_183136699.1 DUF433 domain-containing protein -
  HBB04_RS09140 (HBB04_01855) - 2044792..2045196 (-) 405 WP_005895657.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HBB04_RS09145 (HBB04_01856) pilB 2045423..2047117 (+) 1695 WP_005895655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBB04_RS09150 (HBB04_01857) pilC 2047120..2048337 (+) 1218 WP_024690386.1 type II secretion system F family protein Machinery gene
  HBB04_RS09155 (HBB04_01858) pilD 2048339..2049211 (+) 873 WP_122347408.1 A24 family peptidase Machinery gene
  HBB04_RS09160 (HBB04_01859) coaE 2049208..2049831 (+) 624 WP_122347409.1 dephospho-CoA kinase -
  HBB04_RS09165 (HBB04_01860) yacG 2049828..2050037 (+) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  HBB04_RS09170 (HBB04_01861) - 2050181..2050810 (+) 630 WP_005895647.1 DUF1780 domain-containing protein -
  HBB04_RS09175 (HBB04_01862) - 2050807..2051340 (+) 534 WP_053481610.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62448.69 Da        Isoelectric Point: 6.2549

>NTDB_id=430910 HBB04_RS09145 WP_005895655.1 2045423..2047117(+) (pilB) [Pseudomonas coronafaciens strain X-1]
MTDAVLTGLAKQLVLAELLTETVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDNDSGLGNLEDVDLGLD
IEPADGKETSLAAQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEIDIPRETLIQEGFPESK
IGTFKIFGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=430910 HBB04_RS09145 WP_005895655.1 2045423..2047117(+) (pilB) [Pseudomonas coronafaciens strain X-1]
ATGACTGATGCTGTACTCACTGGCTTGGCCAAACAATTGGTCCTTGCCGAGCTGCTTACCGAAACCGTTGCGCAGCAAGC
GTATCAACAAGCGCGCCGCGACAAAATTTCGTTGGTGAGCTATCTGGTTCAGAACAAGCTGGTCAAAAGCCTGACGCTCG
CAGAGATGGCTTCGGATCAGTTTGGTGTCCCTTTTATGGATCTGGCCAGCCTGGACAAGGAAAGCCAGCCTAAGGGGCTG
GTGAGTGAAAAGCTTATTCGACAGCATCATGCCCTTCCACTATGGCGAAGAGGTAACAAGCTGTTTATCGGTATTTCGGA
CCCGACTAACCATCAGGCGGTGACCGATATTCAGTTCAGCACCGGCCTGAACACTGAAGCGATTCTGGTCGAAGACGACA
AGCTGACCACTGCGATAGACCGTTTTTTTGACAATGACAGCGGGCTGGGCAATCTGGAAGATGTCGATCTGGGTCTGGAT
ATCGAACCCGCTGATGGCAAGGAAACCTCCCTTGCTGCACAGAATGATGCTGACGACGCCCCGGTGGTGCGCTTCGTCAA
CAAAATGCTGTTGGATGCTATCAGGCTAGGATCATCAGACCTGCACTTCGAGCCCTATGAAAAGATCTTTCGTGTGCGCT
TGCGTACCGACGGCATTCTGCACGAAGTCGCCAGGCCGCCGATTCATCTGGCAAACCGTATTGCTGCCCGCTTGAAAGTC
ATGGCCAGCCTCGATATTTCCGAGCGACGTAAACCGCAGGATGGCCGGGTCAAGTTAAGGGTGTCCAAAACAAAGGCCAT
CGATTTCCGTATGAACACCTTGCCCACGCTATGGGGCGAGAAGATCGTGATGCGTATTCTTGATCCGACCAGTGCGCAGA
TGGGCATTGACGCTCTGGGCTATGAACCAGAGCAAAAGGCACTGTATCTGGAGGCACTAAAGCAGCCACAAGGCATGATT
CTTGTGACCGGCCCTACGGGTTCTGGCAAAACTGTTTCCCTGTATACCGGGCTGAATATCCTCAATACTGTGGACATCAA
CATTTCCACCGCTGAAGACCCGGTAGAGATCAACCTTGAAGGCATCAATCAGGTCAACGTCAACCCACGCCAAGGACTCG
ACTTTTCTCAGGCATTGCGCGCCTTTCTGCGTCAAGATCCCGACGTAATCATGGTTGGTGAGATTCGCGACCTGGAGACT
GCAGAAATCGCCATCAAGGCCTCACAGACAGGCCATATGGTGCTTTCAACGCTGCACACCAACAGCGCAGCAGAAACCCT
GACGCGTTTACATCACATGGGCGTCGCTGCATTCAACATTGCCACAGCGATCAACCTGATTATCGCGCAACGACTTGCAC
GCAAACTTTGCAGCCACTGCAAGAAAGAAATCGATATTCCGCGGGAGACGTTGATTCAGGAAGGCTTCCCGGAATCAAAA
ATCGGAACGTTCAAAATCTTTGGCCCCGTGGGCTGCGAACATTGCAACGGCGGCTATAGAGGCCGGGTAGGTATTTACGA
AGTGGTCAAAAAAACAGCAGAGCTGGAACGCATTATCATGGAAGAAGGGAACTCGCTTGAGATATCCAGACAGATGCGCA
AAGACGGTTTCAATGATCTGCGCACTTCGGGCTTGTCAAAAGCGATGCAAGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.951

100

0.582
  pilB Acinetobacter baylyi ADP1

57.067

100

0.573
  pilB Legionella pneumophila strain ERS1305867

55.929

100

0.56
  pilB Vibrio cholerae strain A1552

51.423

99.645

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

51.071

99.291

0.507
  pilB Vibrio campbellii strain DS40M4

49.647

100

0.498
  pilF Neisseria gonorrhoeae MS11

47.865

99.645

0.477
  pilF Thermus thermophilus HB27

38.516

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.783

93.262

0.362