Detailed information NTG0412359

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GTF71_RS01265
• Genome accession: NZ_CP047299
• Coordinates: 271052..272068 (+)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae O1 biovar El Tor strain P27459
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 266052..277068

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTF71_RS01250 (GTF71_01250)	-	266594..267325 (-)	732	WP_000495223.1	glutathione peroxidase	-
GTF71_RS01255 (GTF71_01255)	oxyR	267465..268358 (+)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
GTF71_RS01260 (GTF71_01260)	-	268432..270909 (-)	2478	WP_000668733.1	penicillin-binding protein 1A	-
GTF71_RS01265 (GTF71_01265)	pilM	271052..272068 (+)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
GTF71_RS01270 (GTF71_01270)	pilN	272058..272642 (+)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
GTF71_RS01275 (GTF71_01275)	pilO	272635..273225 (+)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
GTF71_RS01280 (GTF71_01280)	pilP	273215..273733 (+)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
GTF71_RS01285 (GTF71_01285)	pilQ	273759..275474 (+)	1716	WP_000788426.1	type IV pilus secretin PilQ	Machinery gene
GTF71_RS01290 (GTF71_01290)	aroK	275661..276185 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=412359 GTF71_RS01265 WP_000520444.1 271052..272068(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain P27459]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=412359 GTF71_RS01265 WP_000520444.1 271052..272068(+) (pilM) [Vibrio cholerae O1 biovar El Tor strain P27459]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485