Detailed information NTG0409448

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: GPZ88_RS07365
• Genome accession: NZ_CP046919
• Coordinates: 1423946..1426177 (+)
• Length: 743 a.a.
• NCBI ID: WP_166043860.1
• Uniprot ID: A0A6G8I166
• Organism: Streptococcus ruminicola strain CNU_G2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1418946..1431177

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GPZ88_RS07340 (GPZ88_07320)	-	1419691..1421268 (+)	1578	WP_166043852.1	DEAD/DEAH box helicase	-
GPZ88_RS07345 (GPZ88_07325)	-	1421308..1421583 (-)	276	WP_166043854.1	GIY-YIG nuclease family protein	-
GPZ88_RS07350 (GPZ88_07330)	-	1421558..1422319 (-)	762	WP_166043856.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
GPZ88_RS07355 (GPZ88_07335)	-	1422436..1423182 (+)	747	WP_074559556.1	lysophospholipid acyltransferase family protein	-
GPZ88_RS07360 (GPZ88_07340)	-	1423279..1423953 (+)	675	WP_166043858.1	helix-hairpin-helix domain-containing protein	-
GPZ88_RS07365 (GPZ88_07345)	comEC/celB	1423946..1426177 (+)	2232	WP_166043860.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GPZ88_RS07370 (GPZ88_07350)	holA	1426306..1427343 (+)	1038	WP_166043864.1	DNA polymerase III subunit delta	-
GPZ88_RS07375 (GPZ88_07355)	sodA	1427440..1428051 (+)	612	WP_166043866.1	superoxide dismutase SodA	-
GPZ88_RS07380 (GPZ88_07360)	-	1428157..1428882 (+)	726	WP_166043868.1	YebC/PmpR family DNA-binding transcriptional regulator	-
GPZ88_RS07385 (GPZ88_07365)	queA	1428934..1429962 (-)	1029	WP_166043870.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
GPZ88_RS07390 (GPZ88_07370)	-	1430122..1430628 (+)	507	WP_033152227.1	VIT1/CCC1 transporter family protein	-

Sequence

Protein

• Length: 743 a.a.
• Molecular weight: 84124.55 Da
• Isoelectric Point: 9.3105

>NTDB_id=409448 GPZ88_RS07365 WP_166043860.1 1423946..1426177(+) (comEC/celB) [Streptococcus ruminicola strain CNU_G2]
MIKHFPIKPIQLAFLLILLYYFCFEKVLLCLFLLLLALLFLWRHYGWKVWCQISLCLAFFAIFFLTKSNQTEQAYQAAPA
QLSKIQMVPDTISVNGDLLSFRGKEAGQTYQVFYTLKSEKEQKFFKNLNQTMLLSGQVDLEEATPQRNFGGFDYRTYLKH
EGIYRIANLSSITEMRQQSSLSFFERLHELRRQAIVSIQRNFPAPMQHYMTGLLFGYLDKSFDEMSDVYTSLGIIHLFAL
SGMQVGFFVGVFRYFFLRLGLRRDYVDILQIPFSLVYAGLTGFSVSVVRSLIQSAFANLGIKKLDNLAFTLMTLFILMPN
FLLTTGGILSFTYAFILSFIDFDELSHYRKILTECLAISLGSLPVLLYFFSVFQPLSLLLTAIFSLAFDTLILPVLTFVF
LLSPLVKITALNPFFVFLETIIKFSKSLVGTPLVFGRPSLGILLLLLLTIGLLYDCYRHKKVAIFLFSIMALLFFQIKHP
LENEVTVVDIGQGDSIFVRDVKGRTLLIDVGGKVSFSEKNAWQERLTDSNAERTLIPYLNSRGVGKIDQLVLTHTDTDHM
GDMLEVAKQVKIGEVLVSQGSLTKPDFVAKLQAMKTKVRTVSAGDSLPIMGSSLQVLYPIRVGNGSNNDSVVLYGKLLGK
NFLFTGDLEEEGEREVMASYPNLPVDVLKAGHHGSKGSSSPAFLAHISPQIALISAGQNNRYKHPHQETLERFQAQNMTI
YRTDQQGAIRFRGLKTWHIETVR

Nucleotide

• Length: 2232 bp

>NTDB_id=409448 GPZ88_RS07365 WP_166043860.1 1423946..1426177(+) (comEC/celB) [Streptococcus ruminicola strain CNU_G2]
TTGATTAAGCATTTTCCCATTAAGCCAATTCAATTGGCTTTTTTGCTCATTTTACTTTATTATTTTTGTTTTGAAAAAGT
CCTGTTATGCCTATTTTTACTCCTGTTAGCCTTGCTGTTTTTATGGCGACATTACGGGTGGAAAGTTTGGTGTCAGATTT
CACTGTGCTTGGCTTTTTTTGCGATATTTTTCCTGACTAAGTCTAATCAGACAGAACAGGCTTATCAAGCGGCGCCAGCT
CAGCTATCAAAAATCCAGATGGTTCCAGATACGATTTCGGTTAATGGTGATTTGTTATCTTTTCGAGGGAAGGAGGCTGG
GCAGACTTACCAAGTTTTTTATACCTTGAAGTCAGAAAAAGAGCAGAAATTTTTTAAAAATCTTAATCAAACAATGCTTT
TATCAGGGCAGGTGGACTTAGAAGAGGCAACGCCACAGCGGAATTTTGGTGGTTTTGATTACCGCACTTACCTTAAACAT
GAAGGCATTTATCGGATTGCTAATCTTTCTTCGATTACTGAAATGAGACAGCAGAGCTCTTTAAGTTTTTTTGAACGACT
ACATGAACTTAGAAGACAGGCGATTGTCAGTATTCAAAGGAATTTTCCTGCCCCAATGCAACATTATATGACTGGCCTTT
TGTTTGGATATTTGGATAAGTCTTTTGATGAGATGAGTGATGTATACACGAGTTTAGGGATTATTCATCTTTTTGCTCTG
TCTGGTATGCAGGTTGGCTTTTTTGTCGGTGTTTTTCGCTATTTCTTTTTGCGACTTGGCTTAAGGCGTGATTATGTGGA
CATACTGCAAATCCCGTTTTCACTTGTATATGCTGGCTTGACAGGCTTTTCGGTTTCAGTTGTTCGGAGCCTTATTCAGT
CTGCTTTTGCAAATCTTGGCATAAAAAAATTAGATAATCTTGCCTTTACTTTAATGACCCTTTTTATTTTGATGCCAAAT
TTCCTGCTGACAACAGGAGGAATTCTTTCTTTTACCTACGCTTTTATCCTAAGTTTTATTGATTTTGATGAGCTTAGTCA
TTATCGAAAAATCCTTACAGAATGTTTGGCGATTTCGCTGGGAAGTTTACCGGTTTTGCTATATTTCTTCTCAGTATTTC
AACCCTTGTCGCTGCTTTTAACAGCTATTTTTTCACTGGCTTTTGATACGCTTATCTTACCTGTTTTGACTTTTGTTTTT
CTTTTATCACCTCTGGTTAAAATTACCGCGTTAAATCCATTTTTTGTTTTTCTAGAAACTATCATCAAATTTAGCAAGTC
GCTGGTTGGTACCCCACTCGTTTTTGGCAGACCAAGTCTAGGAATTTTGTTGTTATTACTTTTGACAATAGGGCTTCTCT
ATGACTGTTATCGACACAAAAAAGTGGCCATTTTTCTTTTTAGCATAATGGCTTTGCTATTTTTCCAAATCAAGCATCCG
CTCGAAAACGAAGTGACGGTGGTTGACATTGGGCAGGGAGATAGTATTTTTGTGCGTGATGTTAAAGGGCGTACGCTGTT
AATTGATGTCGGAGGAAAAGTGAGTTTTAGTGAGAAGAACGCATGGCAGGAGCGGCTGACGGATAGCAATGCGGAGCGAA
CCTTGATTCCTTATCTCAATAGCCGTGGTGTTGGAAAAATTGACCAGCTGGTTCTGACGCACACGGATACTGACCACATG
GGAGATATGCTGGAAGTGGCAAAGCAGGTGAAAATTGGTGAAGTTTTGGTTAGTCAAGGAAGTTTGACAAAGCCAGATTT
TGTGGCGAAATTACAAGCCATGAAAACCAAGGTGCGCACAGTTTCTGCTGGAGATAGTCTGCCGATTATGGGAAGTAGCT
TGCAGGTGCTGTACCCTATTAGAGTTGGCAATGGTTCTAATAATGATTCGGTGGTGTTGTATGGTAAGCTTCTTGGTAAA
AATTTTCTGTTTACAGGAGATTTGGAAGAAGAGGGCGAGCGTGAGGTGATGGCGTCTTATCCTAATCTGCCAGTTGATGT
TTTAAAAGCAGGACACCATGGCTCAAAAGGGTCATCAAGCCCAGCATTTCTAGCTCATATTTCACCGCAGATTGCTCTGA
TTTCTGCCGGTCAAAATAACCGCTACAAACATCCTCACCAAGAAACGCTTGAACGATTTCAAGCGCAAAATATGACCATT
TACCGCACAGACCAACAAGGCGCCATCCGCTTTCGAGGACTGAAAACTTGGCACATTGAAACAGTGAGGTAA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (491-696)

• Competence protein (Competence) (213-454)

• Domain of unknown function (DUF4131) (DUF4131) (28-173)

3D structure

• AlphaFold DB: A0A6G8I166

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	51.478	100	0.515
comEC/celB	Streptococcus mitis SK321	51.075	100	0.511
comEC/celB	Streptococcus pneumoniae TIGR4	50.604	100	0.507
comEC/celB	Streptococcus pneumoniae Rx1	50.067	100	0.502
comEC/celB	Streptococcus pneumoniae D39	50.067	100	0.502
comEC/celB	Streptococcus pneumoniae R6	50.067	100	0.502
comEC	Lactococcus lactis subsp. cremoris KW2	47.715	100	0.478