Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GCU76_RS02010 Genome accession   NZ_CP045425
Coordinates   375985..376434 (-) Length   149 a.a.
NCBI ID   WP_017696127.1    Uniprot ID   -
Organism   Bacillus subtilis strain JAAA     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 370985..381434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU76_RS01975 yckA 371260..371940 (-) 681 WP_017696121.1 amino acid ABC transporter permease -
  GCU76_RS01980 yckB 371950..372813 (-) 864 WP_072557073.1 transporter substrate-binding domain-containing protein -
  GCU76_RS01985 - 372976..373166 (+) 191 Protein_342 hypothetical protein -
  GCU76_RS01990 - 373211..373522 (+) 312 Protein_343 RDD family protein -
  GCU76_RS01995 yckD 373604..373936 (+) 333 WP_015252849.1 YckD family protein -
  GCU76_RS02000 bglC 374090..375523 (+) 1434 WP_152425339.1 6-phospho-beta-glucosidase -
  GCU76_RS02005 nin/comJ 375560..375958 (-) 399 WP_017696126.1 DNA-entry nuclease inhibitor Regulator
  GCU76_RS02010 nucA/comI 375985..376434 (-) 450 WP_017696127.1 DNA-entry nuclease Machinery gene
  GCU76_RS02015 tlpC 376602..378323 (-) 1722 WP_026113668.1 methyl-accepting chemotaxis protein TlpC -
  GCU76_RS02020 hxlB 378434..378991 (-) 558 WP_017696129.1 6-phospho-3-hexuloisomerase -
  GCU76_RS02025 hxlA 378997..379629 (-) 633 WP_017696130.1 3-hexulose-6-phosphate synthase -
  GCU76_RS02030 hxlR 379861..380223 (+) 363 WP_017696131.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16412.34 Da        Isoelectric Point: 4.5282

>NTDB_id=393958 GCU76_RS02010 WP_017696127.1 375985..376434(-) (nucA/comI) [Bacillus subtilis strain JAAA]
MNITTDIIKTILLVIVIIAAAAAGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=393958 GCU76_RS02010 WP_017696127.1 375985..376434(-) (nucA/comI) [Bacillus subtilis strain JAAA]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGCCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment