Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FIM06_RS08640 Genome accession   NZ_CP041144
Coordinates   1763697..1764131 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain UCMB5044     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1758697..1769131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIM06_RS08635 (FIM06_1636) - 1762197..1763408 (-) 1212 WP_105938241.1 cytochrome P450 -
  FIM06_RS08640 (FIM06_1637) nucA/comI 1763697..1764131 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  FIM06_RS08645 (FIM06_1638) - 1764191..1764946 (+) 756 WP_003154084.1 YoaK family protein -
  FIM06_RS08650 (FIM06_1639) - 1764980..1765342 (-) 363 WP_016936035.1 hypothetical protein -
  FIM06_RS08655 (FIM06_1640) - 1765535..1766863 (-) 1329 WP_105937697.1 S8 family peptidase -
  FIM06_RS08660 (FIM06_1641) - 1767042..1767275 (+) 234 WP_061581358.1 hypothetical protein -
  FIM06_RS08665 (FIM06_1642) - 1767531..1768238 (+) 708 WP_105937696.1 poly-gamma-glutamate hydrolase family protein -
  FIM06_RS08670 (FIM06_1643) - 1768298..1768750 (+) 453 WP_014305039.1 OsmC family protein -
  FIM06_RS08675 (FIM06_1644) - 1768764..1769117 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=369746 FIM06_RS08640 WP_007611576.1 1763697..1764131(+) (nucA/comI) [Bacillus velezensis strain UCMB5044]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=369746 FIM06_RS08640 WP_007611576.1 1763697..1764131(+) (nucA/comI) [Bacillus velezensis strain UCMB5044]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment