Detailed information NTG0356805

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E5A72_RS11880
• Genome accession: NZ_CP039025
• Coordinates: 2438748..2439866 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii ATCC 17978 substr. PMR-High
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2433748..2444866

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5A72_RS11845 (E5A72_11845)	-	2434578..2435426 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
E5A72_RS11850 (E5A72_11850)	-	2435678..2436133 (+)	456	WP_000782976.1	bacteriohemerythrin	-
E5A72_RS11860 (E5A72_11860)	-	2436286..2437353 (+)	1068	WP_004748097.1	hypothetical protein	-
E5A72_RS11865 (E5A72_11865)	-	2437353..2437676 (+)	324	WP_000442590.1	RnfH family protein	-
E5A72_RS11870 (E5A72_11870)	-	2437731..2438129 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
E5A72_RS11875 (E5A72_11875)	fur	2438241..2438678 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
E5A72_RS11880 (E5A72_11880)	pilU	2438748..2439866 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E5A72_RS11885 (E5A72_11885)	pilT	2439894..2440931 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
E5A72_RS11890 (E5A72_11890)	-	2441058..2441750 (+)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=356805 E5A72_RS11880 WP_000347034.1 2438748..2439866(-) (pilU) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=356805 E5A72_RS11880 WP_000347034.1 2438748..2439866(-) (pilU) [Acinetobacter baumannii ATCC 17978 substr. PMR-High]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39