Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW64_RS05675 Genome accession   NZ_CP036052
Coordinates   1094374..1097889 (+) Length   1171 a.a.
NCBI ID   WP_000058642.1    Uniprot ID   -
Organism   Bacillus toyonensis strain JAS03/3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1089374..1102889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW64_RS05645 (EXW64_05755) - 1089846..1090637 (-) 792 WP_098812067.1 phosphotransferase -
  EXW64_RS05650 (EXW64_05760) cspA 1091011..1091214 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  EXW64_RS05655 (EXW64_05765) - 1091352..1091537 (-) 186 WP_000391370.1 hypothetical protein -
  EXW64_RS05660 (EXW64_05770) - 1091828..1092409 (+) 582 WP_000444438.1 competence protein ComK -
  EXW64_RS05665 (EXW64_05780) - 1092918..1093511 (+) 594 WP_000347508.1 TVP38/TMEM64 family protein -
  EXW64_RS05670 (EXW64_05785) lepB 1093694..1094257 (+) 564 WP_000751915.1 signal peptidase I -
  EXW64_RS05675 (EXW64_05790) addB 1094374..1097889 (+) 3516 WP_000058642.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW64_RS05680 (EXW64_05795) addA 1097886..1101611 (+) 3726 WP_000572320.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW64_RS05685 (EXW64_05800) - 1101624..1101911 (+) 288 WP_000179959.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW64_RS05690 (EXW64_05805) gerPF 1102000..1102215 (-) 216 WP_001141566.1 spore germination protein GerPF -
  EXW64_RS05695 (EXW64_05810) - 1102258..1102644 (-) 387 WP_000902321.1 spore germination protein GerPE -
  EXW64_RS05700 (EXW64_05815) gerPD 1102660..1102854 (-) 195 WP_001052804.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134512.81 Da        Isoelectric Point: 5.1460

>NTDB_id=346271 EXW64_RS05675 WP_000058642.1 1094374..1097889(+) (addB) [Bacillus toyonensis strain JAS03/3]
MSLRLVIGRAGSGKSTLCLREVQEALKQRPRGKTILYLVPEQMTFQTQQALIESEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETHYEARPNEKYYDEASVTISTAANL
RAEVEGVAREIRRLVADETYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEMMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDIEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVIGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTTNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVDGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=346271 EXW64_RS05675 WP_000058642.1 1094374..1097889(+) (addB) [Bacillus toyonensis strain JAS03/3]
ATGTCACTTCGATTGGTGATTGGTAGAGCGGGAAGTGGAAAGAGTACACTTTGTTTACGAGAAGTGCAAGAAGCGTTAAA
ACAGCGCCCGAGAGGCAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGAAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCTTGGAAGGTGTTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCAGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCTGAGCAAAAGTTATTAGCG
AATAAAGTATATGATTTACAGCTATTATATGATGATTTTGAACGTGCTTTAATCGGGAAATATTTAGATTCAGAAGACTA
TTTACAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAGTATGTAAATGGTGCTGAAATTTATATAGATGGGTTTCATTCAT
TTTCTCCGCAAGAACTTGAAATTGTAAGACAGCTCATGATTTGTGGAGCGAGAGTCACAATTACGTTAACGATAGATGAA
AAAACATTAGCGCAGCCAGTGAATGAACTGGATTTATTTTATGAAACGACGTTAACATATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGTTTTCATTCTCCGGCATTAGCTCATT
TAGAAACGCATTATGAAGCGCGTCCGAATGAAAAGTATTATGATGAAGCAAGTGTAACAATTAGTACAGCGGCGAATTTA
CGAGCTGAAGTAGAAGGGGTTGCTCGTGAAATTCGTAGACTTGTCGCGGATGAAACGTATCGTTATCGAGATATTGCAGT
ACTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATTCCACACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCATTAGTAGAGTGTATTCGTTCTGCCTTAGAAATTATTAGCGGAAATTGGCGTTAT
GACGCGGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGGAAAGAAATGATGCGTGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGTT
ACCGTTCACTTGACGATACAAACGGAATGATAACAGATATTGAACGTGAAATGGAAGAGAAAATAAACAGGCTGCGTGAT
GTTGTAAGAACGCCAGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCGGGAACAGTGATGCAAATGTGCGAAGCGGTTTA
CTTATTTTTAGAAGAACTTGACGTTCCAAAGAAGTTAGAAGAATTACGTATACGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCAACAGACCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAAATGCTTGGTGAGGAGAAA
ATGTCACTATCTATGTTCACGGACGTTATGTCGACAGGACTTGAGGCACTTCAATTCGCTAACATTCCGCCATCATTAGA
TCAAGTATTAGTTGCCAATATTGATCACTCCAGGTTATCAGATGTGAAAGCTACGTTTATTATCGGCGTAAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGAATTGAACTC
GCGCCAACGACGAGACAAACTTTATTAGAAGAGCAGTTCGTTATGTATCAGATGGTAACGAGAGCGTCTGAGAAATTATA
TATTTCATGCCCACTTGCAGATGAAGAAGGAAAGACATTACTTGCATCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGACTCGTTTATTACGAATGACGTAAACGACTTATCGCGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTGACGTTATCTTATGTCATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGAAATCTAGATTTTTGGTG
GGACGTCTATAACTTCTACGTCACTTCGGATGAATGGAAACAAAAAAGTAGTCGCGTGTTATCGAGTTTATTCTATCGAA
ATCGTGCGCAGAAACTAAGTACGGTAGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
CTCTTCAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTAGATGC
ACCAGATATCGGTGAACTATTCCATGCAGCACTGAAGAGAATTGCAGACAGGCTATTACGCGAAAACCGCACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAAAGACAAATTTTA
TTAAGCTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
CGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAGGTGCCATTCGGTATGGGCGGTACGGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTTATCGGCCGTATTGACCGTGTGGATAAGGCTGAAGATGAAAACGGA
ACATTCCTTCGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTCGTTACTACAAATGCACAAACGTGGATGAAAAAAGGCCACGCAGCATCACCTGCTGGTG
TACTGTACTTTCATATTCATAACCCGATTGTTGAAATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGCCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATTGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTCTGTCAGTTTGATGAATCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGACGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.317

100

0.494

Multiple sequence alignment