Detailed information NTG0333439

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: EM867_RS23485
• Genome accession: NZ_CP034908
• Coordinates: 5079328..5080665 (+)
• Length: 445 a.a.
• NCBI ID: WP_003094694.1
• Uniprot ID: A0A0H2ZG94
• Organism: Pseudomonas aeruginosa strain PA0750
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 5074328..5085665

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EM867_RS23460 (EM867_24710)	pgeF	5074471..5075199 (-)	729	WP_003112833.1	peptidoglycan editing factor PgeF	-
EM867_RS23465 (EM867_24715)	rluD	5075196..5076158 (-)	963	WP_003094686.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
EM867_RS23470 (EM867_24720)	-	5076304..5077329 (+)	1026	WP_003102590.1	outer membrane protein assembly factor BamD	-
EM867_RS23475 (EM867_24725)	-	5077486..5077731 (+)	246	WP_003102591.1	PP0621 family protein	-
EM867_RS23480 (EM867_24730)	pilS	5077721..5079313 (+)	1593	WP_003094692.1	two-component system sensor histidine kinase PilS	Regulator
EM867_RS23485 (EM867_24735)	pilR	5079328..5080665 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
EM867_RS23490 (EM867_24740)	thiO	5080711..5081805 (-)	1095	WP_010895678.1	glycine oxidase ThiO	-
EM867_RS23495 (EM867_24745)	-	5081940..5082449 (+)	510	WP_003112829.1	Tfp pilus assembly protein FimT/FimU	-
EM867_RS23500 (EM867_24750)	-	5082555..5083061 (+)	507	WP_003102598.1	Tfp pilus assembly protein FimT/FimU	-
EM867_RS23505 (EM867_24755)	pilV	5083052..5083609 (+)	558	WP_003119423.1	type 4a pilus minor pilin PilV	Machinery gene
EM867_RS23510 (EM867_24760)	pilW	5083606..5084430 (+)	825	WP_003119422.1	type 4a pilus minor pilin PilW	-
EM867_RS23515 (EM867_24765)	pilX	5084427..5085014 (+)	588	WP_003112826.1	type 4a pilus minor pilin PilX	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=333439 EM867_RS23485 WP_003094694.1 5079328..5080665(+) (pilR) [Pseudomonas aeruginosa strain PA0750]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=333439 EM867_RS23485 WP_003094694.1 5079328..5080665(+) (pilR) [Pseudomonas aeruginosa strain PA0750]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: A0A0H2ZG94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384