Detailed information NTG0033184

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SPJ_RS10190
• Genome accession: NC_012466
• Coordinates: 1912782..1913243 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250539.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae JJA
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1907782..1918243

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPJ_RS10160 (SPJ_2047)	-	1908094..1908918 (-)	825	WP_000727909.1	membrane protein insertase YidC	-
SPJ_RS10165 (SPJ_2048)	rnpA	1908893..1909264 (-)	372	WP_000739246.1	ribonuclease P protein component	-
SPJ_RS13615 (SPJ_2049)	-	1909281..1909412 (-)	132	WP_000768904.1	hypothetical protein	-
SPJ_RS10170 (SPJ_2050)	-	1909413..1910603 (-)	1191	WP_000167771.1	acetate kinase	-
SPJ_RS10175 (SPJ_2051)	comYH	1910654..1911607 (-)	954	WP_000345122.1	class I SAM-dependent methyltransferase	Machinery gene
SPJ_RS10180 (SPJ_2052)	-	1911668..1912255 (-)	588	WP_000679779.1	class I SAM-dependent methyltransferase	-
SPJ_RS10185 (SPJ_2053)	comGG/cglG	1912358..1912804 (-)	447	WP_001811371.1	competence type IV pilus minor pilin ComGG	Machinery gene
SPJ_RS10190 (SPJ_2054)	comGF/cglF	1912782..1913243 (-)	462	WP_000250539.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPJ_RS10195 (SPJ_2055)	comGE/cglE	1913206..1913508 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
SPJ_RS10200 (SPJ_2056)	comGD/cglD	1913471..1913875 (-)	405	WP_000588030.1	competence type IV pilus minor pilin ComGD	Machinery gene
SPJ_RS10205 (SPJ_2057)	comGC/cglC	1913868..1914194 (-)	327	WP_000738625.1	competence type IV pilus major pilin ComGC	Machinery gene
SPJ_RS10210 (SPJ_2058)	comGB/cglB	1914196..1915212 (-)	1017	WP_078134418.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPJ_RS10215 (SPJ_2059)	comGA/cglA/cilD	1915160..1916101 (-)	942	WP_000249573.1	competence type IV pilus ATPase ComGA	Machinery gene
SPJ_RS10220 (SPJ_2060)	-	1916177..1916542 (-)	366	WP_000286415.1	DUF1033 family protein	-
SPJ_RS10225 (SPJ_2061)	-	1916693..1917751 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17851.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=33184 SPJ_RS10190 WP_000250539.1 1912782..1913243(-) (comGF/cglF) [Streptococcus pneumoniae JJA]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=33184 SPJ_RS10190 WP_000250539.1 1912782..1913243(-) (comGF/cglF) [Streptococcus pneumoniae JJA]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae D39	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae R6	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae TIGR4	94.118	100	0.941
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915