Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EEB07_RS11725 Genome accession   NZ_CP033576
Coordinates   2273915..2274352 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain NY12-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2268915..2279352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEB07_RS11700 (EEB07_11700) - 2269936..2271071 (+) 1136 Protein_2234 zinc-dependent alcohol dehydrogenase -
  EEB07_RS11705 (EEB07_11705) - 2271086..2271520 (+) 435 WP_014416871.1 RDD family protein -
  EEB07_RS11710 (EEB07_11710) - 2271592..2271915 (+) 324 WP_007409357.1 YckD family protein -
  EEB07_RS11715 (EEB07_11715) - 2272019..2273455 (+) 1437 WP_014416872.1 family 1 glycosylhydrolase -
  EEB07_RS11720 (EEB07_11720) nin/comJ 2273496..2273894 (-) 399 WP_017419379.1 competence protein ComJ Regulator
  EEB07_RS11725 (EEB07_11725) nucA/comI 2273915..2274352 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  EEB07_RS11730 (EEB07_11730) hxlB 2274683..2275240 (-) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  EEB07_RS11735 (EEB07_11735) hxlA 2275237..2275872 (-) 636 WP_123117601.1 3-hexulose-6-phosphate synthase -
  EEB07_RS11740 (EEB07_11740) - 2276104..2276466 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=324064 EEB07_RS11725 WP_003156588.1 2273915..2274352(-) (nucA/comI) [Bacillus velezensis strain NY12-2]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=324064 EEB07_RS11725 WP_003156588.1 2273915..2274352(-) (nucA/comI) [Bacillus velezensis strain NY12-2]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGTTACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment