Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BCB4264_RS20210 Genome accession   NC_011725
Coordinates   4022298..4022732 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus B4264     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4017298..4027732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCB4264_RS20190 (BCB4264_A4106) - 4018475..4019032 (+) 558 WP_000062062.1 PadR family transcriptional regulator -
  BCB4264_RS20200 (BCB4264_A4108) metE 4019545..4021833 (+) 2289 WP_001007610.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BCB4264_RS20205 (BCB4264_A4109) comJ 4021872..4022261 (-) 390 WP_000424030.1 competence protein ComJ -
  BCB4264_RS20210 (BCB4264_A4110) nucA/comI 4022298..4022732 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  BCB4264_RS20215 (BCB4264_A4111) - 4022959..4023708 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  BCB4264_RS20220 (BCB4264_A4112) kinB 4023747..4025021 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  BCB4264_RS20225 (BCB4264_A4114) - 4025332..4025532 (+) 201 WP_000929280.1 hypothetical protein -
  BCB4264_RS20230 (BCB4264_A4115) - 4025655..4026818 (+) 1164 WP_000434577.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=32086 BCB4264_RS20210 WP_000811494.1 4022298..4022732(-) (nucA/comI) [Bacillus cereus B4264]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=32086 BCB4264_RS20210 WP_000811494.1 4022298..4022732(-) (nucA/comI) [Bacillus cereus B4264]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment