Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D9M09_RS02125 Genome accession   NZ_CP033019
Coordinates   455057..456100 (-) Length   347 a.a.
NCBI ID   WP_070312390.1    Uniprot ID   -
Organism   Janthinobacterium agaricidamnosum strain BHSEK     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450057..461100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9M09_RS02110 (D9M09_02110) - 451134..453242 (-) 2109 WP_121668471.1 EAL domain-containing protein -
  D9M09_RS02115 (D9M09_02115) - 453427..453912 (-) 486 WP_121668472.1 glutathione peroxidase -
  D9M09_RS02120 (D9M09_02120) pilU 453914..455053 (-) 1140 WP_205602324.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  D9M09_RS02125 (D9M09_02125) pilT 455057..456100 (-) 1044 WP_070312390.1 type IV pilus twitching motility protein PilT Machinery gene
  D9M09_RS02130 (D9M09_02130) - 456171..456872 (+) 702 WP_070290071.1 YggS family pyridoxal phosphate-dependent enzyme -
  D9M09_RS02135 (D9M09_02135) proC 456889..457719 (+) 831 WP_121668473.1 pyrroline-5-carboxylate reductase -
  D9M09_RS02140 (D9M09_02140) - 457732..458448 (-) 717 WP_121668474.1 anti-sigma factor -
  D9M09_RS02145 (D9M09_02145) - 458445..459011 (-) 567 WP_070290077.1 sigma-70 family RNA polymerase sigma factor -
  D9M09_RS02150 (D9M09_02150) - 459102..459659 (-) 558 WP_070312386.1 DUF3455 domain-containing protein -
  D9M09_RS02155 (D9M09_02155) - 459749..460096 (-) 348 WP_121668475.1 hypothetical protein -
  D9M09_RS02160 (D9M09_02160) - 460093..460470 (-) 378 WP_083287244.1 phage holin family protein -
  D9M09_RS02165 (D9M09_02165) - 460525..460881 (-) 357 WP_070290084.1 DUF883 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38047.52 Da        Isoelectric Point: 6.6378

>NTDB_id=320490 D9M09_RS02125 WP_070312390.1 455057..456100(-) (pilT) [Janthinobacterium agaricidamnosum strain BHSEK]
MDISELLAFSVSNKASDLHLSSGLPPMIRVNGDVRRLNVAPLEHKEVHSMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEDLNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTKDGAGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGSNVGMQTLDQC
LSDLVRRGTISAETARSAAKSPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=320490 D9M09_RS02125 WP_070312390.1 455057..456100(-) (pilT) [Janthinobacterium agaricidamnosum strain BHSEK]
ATGGACATCTCCGAACTTCTCGCTTTCTCCGTCAGCAACAAGGCTTCCGACCTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGGGTCAATGGCGACGTGCGCCGCCTCAACGTGGCGCCGCTCGAGCACAAGGAAGTGCACAGCATGATCTACGACA
TCATGAACGACAGCCAGCGCAAGGCGTATGAAGAGGCGCTCGAGTGCGATTTCTCGTTCGAAATTCCCGGCCTGGCCCGC
TTCCGCGTCAACGCCTACAACCAGGAACGCGGCGCCTCGGCCGTGCTGCGCACGATTCCGTCGAAAGTGCTGACCCTGGA
AGACCTGAACGCGCCGCGCATCTTCGGCGAACTGGCCATGCGCCCGCGGGGCCTCGTGCTGGTGACGGGCCCGACGGGTT
CGGGCAAGTCGACCACCCTGGCAGCGATGGTGAACCACGTCAACGAGCGCCTGAATCACCATATTCTGACCATCGAAGAC
CCGATCGAATTCGTGCATGAGCCGAAGAAATGCCTGATCAACCAGCGCGAGGTAGGCTCGCACACGCATTCGTTCAGCAA
TGCGCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTCATTCTGGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAAACGGGCCACCTGGTGTTCGGCACCCTGCATACCTCGTCGGCGGCAAAATCGATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAGTCCTTGCAAGCCGTCATTTCGCAGAA
CTTGCTCAAAACCAAGGACGGCGCTGGGCGCGTGGCCGCGCATGAAATCATGCTGGCCACGCCGGCCGTGCGCAACCTGA
TCCGCGAAGCGAAAGTGGCGCAAATGTATTCCGCCATCCAGACGGGCAGCAACGTGGGCATGCAGACGCTGGACCAGTGC
CTGTCCGACCTCGTGCGGCGCGGCACGATCTCGGCGGAAACTGCCCGCTCGGCCGCCAAATCGCCCGAAAACTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

71.681

97.695

0.7
  pilT Legionella pneumophila strain ERS1305867

71.681

97.695

0.7
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Pseudomonas stutzeri DSM 10701

41.739

99.424

0.415
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

40.58

99.424

0.403

Multiple sequence alignment