Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D1108_RS06320 Genome accession   NZ_CP031859
Coordinates   1413759..1414307 (-) Length   182 a.a.
NCBI ID   WP_237593297.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain HS143     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1408759..1419307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1108_RS06305 (D1108_06350) - 1409241..1409762 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  D1108_RS06310 (D1108_06355) - 1409867..1411153 (+) 1287 WP_237593295.1 NAD(P)/FAD-dependent oxidoreductase -
  D1108_RS06315 (D1108_06360) - 1411345..1413684 (-) 2340 WP_237593296.1 Tex family protein -
  D1108_RS06320 (D1108_06365) pilF 1413759..1414307 (-) 549 WP_237593297.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  D1108_RS06325 (D1108_06370) - 1414361..1415542 (-) 1182 WP_005608495.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  D1108_RS06350 (D1108_06395) gltX 1416447..1417886 (+) 1440 WP_237592596.1 glutamate--tRNA ligase -
  D1108_RS06360 (D1108_06405) yefM 1418224..1418475 (+) 252 WP_017357689.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  D1108_RS06365 (D1108_06410) - 1418472..1418732 (+) 261 WP_237593298.1 Txe/YoeB family addiction module toxin -
  D1108_RS06370 (D1108_06415) - 1418891..1419058 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20643.25 Da        Isoelectric Point: 6.6300

>NTDB_id=312003 D1108_RS06320 WP_237593297.1 1413759..1414307(-) (pilF) [Actinobacillus pleuropneumoniae strain HS143]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPNAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=312003 D1108_RS06320 WP_237593297.1 1413759..1414307(-) (pilF) [Actinobacillus pleuropneumoniae strain HS143]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAACGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATCGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445

Multiple sequence alignment