Detailed information NTG0030794

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: NPUN_RS25310
• Genome accession: NC_010628
• Coordinates: 6201052..6202158 (+)
• Length: 368 a.a.
• NCBI ID: WP_012411304.1
• Uniprot ID: B2J1B8
• Organism: Nostoc punctiforme PCC 73102
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6196052..6207158

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NPUN_RS25285 (Npun_R5000)	-	6196374..6196886 (-)	513	WP_012411299.1	TIGR02652 family protein	-
NPUN_RS25290 (Npun_F5001)	-	6197127..6197654 (+)	528	WP_012411300.1	gamma carbonic anhydrase family protein	-
NPUN_RS25295 (Npun_F5002)	-	6197922..6198047 (+)	126	WP_012411301.1	photosystem II protein Y	-
NPUN_RS25300 (Npun_F5003)	-	6198265..6199563 (+)	1299	WP_041565611.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-
NPUN_RS25305 (Npun_R5004)	-	6199600..6200898 (-)	1299	WP_012411303.1	ABC transporter substrate-binding protein	-
NPUN_RS25310 (Npun_F5005)	pilM	6201052..6202158 (+)	1107	WP_012411304.1	type IV pilus assembly protein PilM	Machinery gene
NPUN_RS25315 (Npun_F5006)	-	6202266..6203042 (+)	777	WP_012411305.1	PilN domain-containing protein	-
NPUN_RS25320 (Npun_F5007)	-	6203039..6203815 (+)	777	WP_012411306.1	pilus assembly protein PilO	-
NPUN_RS25325 (Npun_F5008)	-	6203952..6206330 (+)	2379	WP_012411307.1	type IV pilus secretin family protein	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 40652.68 Da
• Isoelectric Point: 4.4980

>NTDB_id=30794 NPUN_RS25310 WP_012411304.1 6201052..6202158(+) (pilM) [Nostoc punctiforme PCC 73102]
MVQKFNRLFGKSNKGVGIELAPERVNVVQLRKQRQGLKLETFTSVAVPEGVVTDGQITDPAAMAQLIQQALAESKIKTTR
VATGVPGRDSIVRIIPVPTELDDKELREMVLNHEAGLYLPYPREEADVDYQKLDYFVDEDGIEKVHVLLVATRKEITDTY
ISTFEQAGLQIDVLEINSFALIRTIREQLRQFGPQEAAVLVDIEFDSTEIAIIVNGVPQFSRTVPIGTYQMQTALARAMS
LPTSRDMELLHGMIIPPTPLDGGKTGVTEIDPGMAAILRVLGELTDELRRSIDFYLNQSENLEVAQILLAGPGGGLQQLD
EFFTQRLSLPTTQIDPIGSLSLEVDTDKYPQVKRSGLAIVLGLGMREV

Nucleotide

• Length: 1107 bp

>NTDB_id=30794 NPUN_RS25310 WP_012411304.1 6201052..6202158(+) (pilM) [Nostoc punctiforme PCC 73102]
GTGGTACAAAAATTCAATCGTCTGTTTGGCAAATCGAATAAAGGGGTCGGCATTGAACTTGCTCCCGAACGGGTAAATGT
AGTTCAGCTACGCAAACAGCGTCAAGGCTTGAAACTAGAAACCTTTACATCGGTAGCAGTTCCAGAAGGCGTAGTTACCG
ATGGTCAAATCACTGATCCCGCAGCAATGGCGCAATTAATCCAACAGGCGCTAGCTGAAAGCAAAATCAAAACTACTCGT
GTTGCCACTGGTGTACCAGGGCGAGATTCCATCGTTCGGATTATACCTGTACCAACGGAGTTAGATGACAAAGAACTGCG
AGAAATGGTGCTAAACCATGAAGCAGGTTTGTATTTACCCTATCCCCGTGAAGAAGCTGATGTAGATTATCAAAAACTTG
ACTACTTTGTAGATGAGGATGGCATTGAAAAAGTACATGTACTTCTAGTAGCCACTCGCAAGGAAATAACAGATACCTAT
ATAAGTACATTCGAGCAGGCAGGACTACAAATTGATGTTTTAGAAATTAACAGTTTTGCCCTAATTCGGACTATTCGTGA
GCAACTGCGACAATTTGGGCCGCAAGAAGCAGCAGTACTAGTTGATATAGAGTTCGACAGTACAGAAATCGCCATCATTG
TTAACGGAGTACCGCAATTTTCGCGCACAGTGCCCATAGGCACTTATCAAATGCAAACTGCGTTAGCAAGGGCAATGAGC
TTACCCACATCACGAGATATGGAACTGTTACACGGAATGATTATTCCCCCAACTCCTCTAGATGGTGGGAAAACCGGTGT
TACCGAAATCGATCCTGGTATGGCAGCCATATTGAGAGTATTGGGTGAACTAACGGATGAACTGCGCCGTTCGATCGATT
TTTACCTTAATCAAAGTGAAAATTTGGAGGTAGCGCAGATTTTATTAGCTGGGCCAGGAGGCGGACTCCAACAGCTAGAT
GAATTCTTTACCCAACGATTGAGCTTGCCAACTACCCAAATAGATCCAATTGGGTCTTTGTCGTTAGAAGTTGACACTGA
TAAATATCCACAGGTAAAACGCTCTGGCTTGGCGATTGTACTTGGTTTAGGAATGCGGGAGGTGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (283-367)

• Type IV pilus assembly protein PilM; (PilM_2) (17-238)

3D structure

• AlphaFold DB: B2J1B8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Synechocystis sp. PCC 6803	65.94	99.728	0.658