Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   APL_RS01025 Genome accession   NC_009053
Coordinates   219442..220740 (+) Length   432 a.a.
NCBI ID   WP_005613635.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae serovar 5b str. L20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214442..225740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APL_RS01000 (APL_0195) - 214614..217181 (-) 2568 WP_005600249.1 penicillin-binding protein 1A -
  APL_RS01005 (APL_0196) - 217320..218000 (+) 681 WP_009875022.1 hypothetical protein -
  APL_RS01010 (APL_0197) - 217982..218503 (+) 522 WP_005595970.1 hypothetical protein -
  APL_RS01015 (APL_0198) - 218602..219033 (+) 432 WP_009875020.1 hypothetical protein -
  APL_RS01020 (APL_0199) - 219026..219430 (+) 405 WP_005595974.1 hypothetical protein -
  APL_RS01025 (APL_0200) comE 219442..220740 (+) 1299 WP_005613635.1 type IV pilus secretin PilQ Machinery gene
  APL_RS01030 (APL_0201) nusB 220866..221279 (+) 414 WP_005595979.1 transcription antitermination factor NusB -
  APL_RS01035 (APL_0202) thiL 221344..222309 (+) 966 WP_005618774.1 thiamine-phosphate kinase -
  APL_RS01040 (APL_0203) - 222322..222795 (+) 474 WP_005595983.1 phosphatidylglycerophosphatase A -
  APL_RS01045 (APL_0204) - 222804..223430 (+) 627 WP_005595985.1 LysE family transporter -
  APL_RS01050 (APL_0205) - 223489..224526 (-) 1038 WP_005595987.1 TrmH family RNA methyltransferase -
  APL_RS01055 (APL_0206) suhB 224692..225504 (+) 813 WP_005595989.1 inositol-1-monophosphatase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47775.91 Da        Isoelectric Point: 9.4227

>NTDB_id=27610 APL_RS01025 WP_005613635.1 219442..220740(+) (comE) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
MRKMIWLLLFILTPVMANTFSLSLKNAPTAEILSYLAEEYGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTSAY
EKQIYFIGHKKDEKAATAIGVNSDLLKPKLITKTIKLDYAKAAEVIESLTKGSGHFLSENGYLHFDDRSNSLIIKDSPES
MKNIVQLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGIFAPTNGHHKIAGSLEANGLPNTNRLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENNVEIIASPRLLTTNKKPASIKQGTEVPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDK
QILLDLVVTQNSPNSTGSTLQGLVTIDKQELNTQVFAQHGETIVLGGIFQHLTAKGEDRVPILGSIPVLKKLFSHSSDKI
SKRELVIFVTPYIVKNGQPQISSHSSQKLAPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=27610 APL_RS01025 WP_005613635.1 219442..220740(+) (comE) [Actinobacillus pleuropneumoniae serovar 5b str. L20]
ATGCGAAAAATGATTTGGTTGCTCCTGTTTATATTAACGCCGGTTATGGCAAATACTTTTTCTCTCTCGCTGAAAAATGC
GCCGACTGCGGAAATTTTAAGTTATTTAGCCGAAGAATACGGTAAAAATATCGTGTTAAGCGACAATATTGAGACAAATA
CTACATTAAGAATTGAAAATAGTGATTTTGACAGCGTATTAAAAAGTATTACTCGGGCGAATAAACTCACGAGTGCATAC
GAAAAACAGATCTATTTTATCGGGCATAAAAAAGATGAGAAAGCGGCTACGGCAATAGGCGTAAATTCTGATTTATTGAA
ACCTAAATTGATCACTAAGACAATAAAATTAGATTATGCGAAGGCGGCGGAAGTGATTGAATCTTTAACTAAAGGAAGCG
GCCATTTTTTATCGGAAAACGGCTATTTGCATTTTGATGATCGTAGTAACAGTTTGATTATTAAAGACAGTCCGGAATCG
ATGAAAAATATCGTGCAATTAATCAGAAACTTAGATAAACCGACCGAACAGATTGCAATTGAAGCCAGAATTGTCACGAT
AAGCAGTGAGAATTTACAAGAATTAGGTGTGCGTTGGGGGATATTTGCTCCTACAAACGGACACCATAAAATTGCCGGTT
CGCTTGAAGCGAACGGATTACCGAATACTAATCGTTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCACTA
CAAGTGGCGAAAATTAATGGGCGAGTGCTTGATTTGGAACTTACCGCTTTGGAACAAGAAAATAATGTTGAGATTATCGC
CAGCCCTCGTTTATTAACCACCAATAAGAAGCCGGCAAGCATTAAGCAAGGAACGGAAGTTCCTTATGTGCTGTATAACC
GTAAAGACGAAGTGAAAAATATCGAATTTAAAGAAGCGGTTTTAGGTTTACAAGTGACGCCGCATATTTCAAACGATAAG
CAAATTTTGCTCGATTTGGTAGTCACCCAAAACTCACCTAATTCGACCGGTTCAACCCTACAAGGCTTAGTGACGATTGA
TAAGCAGGAACTGAATACGCAAGTATTTGCTCAGCATGGCGAAACGATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTGCCGATTTTAGGTTCGATTCCGGTACTTAAAAAGTTATTTAGCCATTCTAGTGATAAAATT
AGTAAGCGTGAACTAGTGATTTTTGTGACGCCTTACATTGTTAAAAACGGACAACCGCAAATTTCCTCACATTCTTCACA
GAAATTAGCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.506

96.065

0.639
  comE Haemophilus influenzae Rd KW20

53.037

99.074

0.525
  comE Haemophilus influenzae 86-028NP

52.804

99.074

0.523
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

96.065

0.407
  pilQ Vibrio cholerae strain A1552

42.41

96.065

0.407
  pilQ Vibrio campbellii strain DS40M4

40.326

99.306

0.4

Multiple sequence alignment