Detailed information NTG0240696

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CF389_RS02410
• Genome accession: NZ_CP022556
• Coordinates: 449864..450478 (+)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain NJAU-Z9
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 444864..455478

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CF389_RS02375	aroE	445551..446393 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
CF389_RS02380	yhbY	446387..446677 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CF389_RS02385	-	446688..447257 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
CF389_RS02390	yqeK	447247..447828 (+)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CF389_RS02395	rsfS	447825..448181 (+)	357	WP_003152864.1	ribosome silencing factor	-
CF389_RS02400	-	448178..448915 (+)	738	WP_003152865.1	class I SAM-dependent DNA methyltransferase	-
CF389_RS02405	comER	448984..449805 (-)	822	WP_003152866.1	late competence protein ComER	-
CF389_RS02410	comEA	449864..450478 (+)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
CF389_RS02415	-	450545..451114 (+)	570	WP_003152868.1	ComE operon protein 2	-
CF389_RS02420	comEC	451115..453466 (+)	2352	WP_003152869.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CF389_RS02425	-	453485..453619 (-)	135	WP_003152870.1	YqzM family protein	-
CF389_RS19300	-	453660..453814 (+)	155	Protein_454	hypothetical protein	-
CF389_RS02430	holA	453854..454895 (+)	1042	Protein_455	DNA polymerase III subunit delta	-
CF389_RS02435	rpsT	454912..455178 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=240696 CF389_RS02410 WP_003152867.1 449864..450478(+) (comEA) [Bacillus velezensis strain NJAU-Z9]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=240696 CF389_RS02410 WP_003152867.1 449864..450478(+) (comEA) [Bacillus velezensis strain NJAU-Z9]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363