Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B9L64_RS05175 Genome accession   NZ_CP020915
Coordinates   1037297..1037707 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain 50-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1032297..1042707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9L64_RS05145 - 1032384..1032524 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  B9L64_RS05150 yqeF 1032830..1033561 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  B9L64_RS05155 cwlH 1033813..1034565 (-) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  B9L64_RS05160 yqeD 1034752..1035378 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  B9L64_RS05165 gnd 1035397..1036290 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  B9L64_RS05170 yqeB 1036542..1037264 (+) 723 WP_010886572.1 hypothetical protein -
  B9L64_RS05175 nucA/comI 1037297..1037707 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  B9L64_RS05180 sigK 1037903..1038631 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  B9L64_RS20965 - 1038631..1038747 (+) 117 WP_122894874.1 hypothetical protein -
  B9L64_RS05185 - 1038713..1038909 (-) 197 Protein_1037 recombinase family protein -
  B9L64_RS05190 - 1038867..1039031 (-) 165 WP_080265384.1 hypothetical protein -
  B9L64_RS05195 - 1039464..1040057 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  B9L64_RS05200 - 1040215..1041210 (+) 996 WP_029318099.1 acryloyl-CoA reductase -
  B9L64_RS05205 - 1041538..1041771 (+) 234 Protein_1041 helix-turn-helix domain-containing protein -
  B9L64_RS05210 arsC 1041982..1042401 (-) 420 WP_029318100.1 arsenate reductase (thioredoxin) -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=227070 B9L64_RS05175 WP_009967785.1 1037297..1037707(-) (nucA/comI) [Bacillus subtilis strain 50-1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=227070 B9L64_RS05175 WP_009967785.1 1037297..1037707(-) (nucA/comI) [Bacillus subtilis strain 50-1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment