Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   BSR25_RS03110 Genome accession   NZ_CP020604
Coordinates   601011..602333 (-) Length   440 a.a.
NCBI ID   WP_021214546.1    Uniprot ID   A0A2X0PIL8
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 596011..607333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR25_RS03080 (BSR25_0605) - 597154..598122 (+) 969 WP_003130649.1 PhoH family protein -
  BSR25_RS03085 (BSR25_0606) - 598180..598659 (+) 480 WP_003130650.1 NUDIX hydrolase -
  BSR25_RS03090 (BSR25_0607) ybeY 598811..599299 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  BSR25_RS03095 (BSR25_0608) - 599283..599738 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  BSR25_RS03100 (BSR25_0609) - 599752..600330 (+) 579 WP_003130655.1 nucleotidyltransferase family protein -
  BSR25_RS13265 comFC 600364..600768 (-) 405 WP_003130657.1 ComF family protein Machinery gene
  BSR25_RS03110 (BSR25_0611) comFA 601011..602333 (-) 1323 WP_021214546.1 DEAD/DEAH box helicase Machinery gene
  BSR25_RS03115 (BSR25_0612) - 602390..603019 (+) 630 WP_021214545.1 YigZ family protein -
  BSR25_RS03120 (BSR25_0613) - 603153..603650 (+) 498 WP_003130662.1 VanZ family protein -
  BSR25_RS03125 (BSR25_0614) - 603698..604717 (+) 1020 WP_003130663.1 DUF475 domain-containing protein -
  BSR25_RS03130 (BSR25_0615) - 604827..605579 (+) 753 WP_010905753.1 bifunctional metallophosphatase/5'-nucleotidase -
  BSR25_RS03135 (BSR25_0616) glyQ 605864..606817 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50187.69 Da        Isoelectric Point: 10.1065

>NTDB_id=224901 BSR25_RS03110 WP_021214546.1 601011..602333(-) (comFA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDDFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=224901 BSR25_RS03110 WP_021214546.1 601011..602333(-) (comFA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain FM03]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGATTTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X0PIL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

38.018

98.636

0.375

Multiple sequence alignment