Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BUM85_RS10345 Genome accession   NZ_CP018842
Coordinates   2033618..2034991 (-) Length   457 a.a.
NCBI ID   WP_002458651.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain 14.1.R1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2028618..2039991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUM85_RS10330 (BUM85_10330) cysE 2029604..2030233 (-) 630 WP_172952487.1 serine O-acetyltransferase -
  BUM85_RS10335 (BUM85_10335) gltX 2030642..2032096 (-) 1455 WP_002475525.1 glutamate--tRNA ligase -
  BUM85_RS10340 (BUM85_10340) - 2032506..2033564 (-) 1059 WP_002475537.1 PIN/TRAM domain-containing protein -
  BUM85_RS10345 (BUM85_10345) radA 2033618..2034991 (-) 1374 WP_002458651.1 DNA repair protein RadA Machinery gene
  BUM85_RS10350 (BUM85_10350) - 2035698..2038151 (-) 2454 WP_002458652.1 ATP-dependent Clp protease ATP-binding subunit -
  BUM85_RS10355 (BUM85_10355) - 2038165..2039172 (-) 1008 WP_080035617.1 protein arginine kinase -
  BUM85_RS10360 (BUM85_10360) - 2039162..2039725 (-) 564 WP_002475503.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50242.84 Da        Isoelectric Point: 7.5278

>NTDB_id=210942 BUM85_RS10345 WP_002458651.1 2033618..2034991(-) (radA) [Staphylococcus epidermidis strain 14.1.R1]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKVAHPKHGVKTKETAGKVQKLNSVKHEATPRLLTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICAALSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
EERPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDQAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPQTNIGGWTFPEGIQVVGVSSVHEALKYALHSKPR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=210942 BUM85_RS10345 WP_002458651.1 2033618..2034991(-) (radA) [Staphylococcus epidermidis strain 14.1.R1]
ATGGCCAAGAAAAAAGTAATATTCGAATGTATGGCATGTGGCTATCAATCTCCTAAGTGGATGGGTAAGTGCCCTAACTG
CGGCGCGTGGAATCAAATGGAAGAAATAGTAGAAAAGGTTGCTCATCCTAAACATGGTGTAAAAACAAAAGAAACAGCAG
GTAAGGTACAAAAGTTAAATAGTGTTAAGCATGAAGCTACCCCTAGACTACTTACAGAATCAAAGGAATTTAACCGCGTT
TTAGGTGGAGGTATTGTAAGTGGTTCATTAGTACTAATAGGTGGAGACCCAGGTATTGGTAAGTCGACTTTATTGCTACA
AATTTGTGCTGCGCTGTCACAAAATAAGAATGTTTTATATATTACTGGTGAGGAATCTATTAATCAAACTAAGTTGCGCG
CTGATCGTTTGGAAGAAGATTCTAGTCATCTTAATGTTTTAGCCGAAACTGATTTAGAAGTGATACATCAAACTGTAAAA
GAAGAGAGACCTGACTTACTTGTTGTTGATTCGATTCAAACAATCTATCATCCGGAAATTAGTTCTGCACCTGGATCGGT
ATCACAAGTAAGAGAGAGTACACAAAGTTTAATGAACATTGCTAAACAGATGAATATTGCCACATTTATTGTGGGACACG
TAACAAAAGAAGGACAAATCGCCGGACCAAGATTATTGGAACATATGGTTGATACAGTTCTTTATTTTGAAGGAGACGAG
CATCACGCTTATCGTATCCTTAGAGCAGTAAAAAATAGATTTGGTTCTACAAATGAGATGGGGATTTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTACTTAATCCTTCTGAAATGTTTTTAGAGGAACGTTCTACAAATGTTCCGGGCTCTACAATCG
TCCCCACTATGGAAGGAACAAGACCACTACTCATTGAAGTCCAAGCGCTTGTTACACCAACTACATTTAATAATCCTAGA
CGAATGGCTACAGGTATAGATCATAATCGATTAAGTTTACTTATGGCGGTTCTAGAAAAAAAGGAAAACTATTTACTCCA
ACAACAAGATGCCTATATTAAAGTAGCAGGTGGCGTCAAATTAACAGAACCTGCTGTTGATTTAAGTATTATTGTTGCGA
CAGCATCAAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTGTGGGTGAAGTTGGATTAACAGGTGAAGTACGC
AGAGTATCTCGCATAGAGCAACGTGTTCAAGAAGCGGCCAAACTAGGGTTTAAAAGAGCTATTATTCCACAGACAAATAT
TGGAGGTTGGACATTCCCAGAAGGCATCCAAGTCGTTGGTGTTTCATCAGTACATGAAGCTTTAAAATATGCATTACATT
CAAAACCGAGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.231

100

0.665
  radA Streptococcus pneumoniae Rx1

60.802

98.249

0.597
  radA Streptococcus pneumoniae D39

60.802

98.249

0.597
  radA Streptococcus pneumoniae R6

60.802

98.249

0.597
  radA Streptococcus pneumoniae TIGR4

60.802

98.249

0.597
  radA Streptococcus mitis NCTC 12261

60.802

98.249

0.597
  radA Streptococcus mitis SK321

60.802

98.249

0.597

Multiple sequence alignment