Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BI197_RS12155 Genome accession   NZ_CP017747
Coordinates   2537789..2538226 (+) Length   145 a.a.
NCBI ID   WP_071392144.1    Uniprot ID   -
Organism   Bacillus velezensis strain SYBC H47     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2532789..2543226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI197_RS12140 - 2535645..2536007 (-) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -
  BI197_RS12145 hxlA 2536239..2536874 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  BI197_RS12150 hxlB 2536871..2537428 (+) 558 WP_071392143.1 6-phospho-3-hexuloisomerase -
  BI197_RS12155 nucA/comI 2537789..2538226 (+) 438 WP_071392144.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BI197_RS12160 comJ 2538249..2538647 (+) 399 Protein_2436 competence protein ComJ -
  BI197_RS19075 - 2538666..2538895 (-) 230 Protein_2437 family 1 glycosylhydrolase -
  BI197_RS20075 - 2538820..2539173 (-) 354 WP_082181748.1 family 1 glycosylhydrolase -
  BI197_RS12170 - 2539115..2539390 (-) 276 WP_373369498.1 YckD family protein -
  BI197_RS12175 - 2539507..2539941 (-) 435 WP_021494203.1 RDD family protein -
  BI197_RS12180 - 2539956..2541092 (-) 1137 WP_071392146.1 zinc-dependent alcohol dehydrogenase -
  BI197_RS12185 - 2541157..2542359 (-) 1203 WP_071392147.1 GTP-binding protein -
  BI197_RS19860 - 2542504..2542632 (+) 129 Protein_2443 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16099.43 Da        Isoelectric Point: 9.4115

>NTDB_id=202465 BI197_RS12155 WP_071392144.1 2537789..2538226(+) (nucA/comI) [Bacillus velezensis strain SYBC H47]
MKTLKALLLVLIIIAGAVAALIKGDLFSNNEQTVRKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=202465 BI197_RS12155 WP_071392144.1 2537789..2538226(+) (nucA/comI) [Bacillus velezensis strain SYBC H47]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAAATAACGAGCAGACCGTTCGGAAGAGTGATTACGATAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGG
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634

Multiple sequence alignment