Detailed information NTG0193053

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BE841_RS08055
• Genome accession: NZ_CP016868
• Coordinates: 1833979..1835043 (-)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila subsp. pneumophila strain FFI102
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1828979..1840043

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE841_RS08030 (BE841_08030)	aroK	1829160..1829687 (-)	528	WP_010946667.1	shikimate kinase AroK	-
BE841_RS08035 (BE841_08035)	pilQ	1830133..1832229 (-)	2097	WP_070322471.1	type IV pilus secretin PilQ	Machinery gene
BE841_RS08040 (BE841_08040)	pilP	1832233..1832817 (-)	585	WP_011215097.1	pilus assembly protein PilP	Machinery gene
BE841_RS08045 (BE841_08045)	pilO	1832814..1833413 (-)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
BE841_RS08050 (BE841_08050)	pilN	1833427..1833975 (-)	549	WP_011215095.1	PilN domain-containing protein	Machinery gene
BE841_RS08055 (BE841_08055)	pilM	1833979..1835043 (-)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
BE841_RS08060 (BE841_08060)	ravI	1835460..1836467 (+)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
BE841_RS08065 (BE841_08065)	-	1836619..1839003 (+)	2385	WP_011215092.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=193053 BE841_RS08055 WP_011215094.1 1833979..1835043(-) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI102]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=193053 BE841_RS08055 WP_011215094.1 1833979..1835043(-) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI102]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407